CCT2 Primary Antibody
|Aliases||CCTB; 99D8.1; PRO1633; CCT-beta; MGC142074; MGC142076; TCP-1-beta|
|Formulation||Ascitic fluid containing 0.03% sodium azide.|
|Immunogen||Purified recombinant fragment of human CCT2 expressed in E. Coli. |
|Shipping Information||This product will ship in a box containing blue ice at a temperature of 4°C. Learn More|
|Species Reactivity||Human, Mouse, Monkey, Rat|
|ICC (Immunocytochemistry)||1/200 - 1/1000|
|FCM (Flow Cytometry)||1/200 - 1/400|
|WB (Western Blot)||1/500 - 1/2000|
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Figure 1: Western blot analysis using CCT2 mAb against human CCT2 (AA: 87-290) recombinant protein. (Expected MW is 47.9 kDa)
Figure 2: Western blot analysis using CCT2 mouse mAb against Hela (1), MCF-7 (2), Jurkat (3), T47D (4), K562 (5), A431 (6), NIH/3T3 (7), PC-12 (8) and Cos7 (9) cell lysate.
Figure 3: Immunofluorescence analysis of 3T3-L1 cells using CCT2 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
Figure 4: Flow cytometric analysis of NIH/3T3 cells using CCT2 mouse mAb (blue) and negative control (red).
Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);
The protein encoded by this gene is a molecular chaperone that is a member of the chaperonin containing TCP1 complex (CCT), also known as the TCP1 ring complex (TRiC). This complex consists of two identical stacked rings, each containing eight different proteins. Unfolded polypeptides enter the central cavity of the complex and are folded in an ATP-dependent manner. The complex folds various proteins, including actin and tubulin. Two transcript variants encoding different isoforms have been found for this gene.
|References (references)||1. J Biol Chem. 2009 May 29;284(22):14939-48. |
2. Mol Cell Proteomics. 2009 Jan;8(1):157-71.