CCT2 Primary Antibody

Item Information

Catalog #

Size

Price

Description

The protein encoded by this gene is a molecular chaperone that is a member of the chaperonin containing TCP1 complex (CCT), also known as the TCP1 ring complex (TRiC). This complex consists of two identical stacked rings, each containing eight different proteins. Unfolded polypeptides enter the central cavity of the complex and are folded in an ATP-dependent manner. The complex folds various proteins, including actin and tubulin. Two transcript variants encoding different isoforms have been found for this gene.

Product Overview

Entrez GenelD

10576

Aliases

CCTB; 99D8.1; PRO1633; CCT-beta; MGC142074; MGC142076; TCP-1-beta

Clone#

5B5C4

Host / Isotype

Mouse / IgG1

Species Reactivity

Human, Mouse, Monkey, Rat

Immunogen

Purified recombinant fragment of human CCT2 expressed in E. Coli.

Formulation

Ascitic fluid containing 0.03% sodium azide.

Storage

4°C; -20°C for long term storage

Product Applications

WB (Western Blot)

1/500 - 1/2000

ICC (Immunocytochemistry)

1/200 - 1/1000

FCM (Flow Cytometry)

1/200 - 1/400

ELISA

1/10000

References

1. J Biol Chem. 2009 May 29;284(22):14939-48.
2. Mol Cell Proteomics. 2009 Jan;8(1):157-71.

Product Image

Western Blot

Figure 1: Western blot analysis using CCT2 mAb against human CCT2 (AA: 87-290) recombinant protein. (Expected MW is 47.9 kDa)

Western Blot

Figure 2: Western blot analysis using CCT2 mouse mAb against Hela (1), MCF-7 (2), Jurkat (3), T47D (4), K562 (5), A431 (6), NIH/3T3 (7), PC-12 (8) and Cos7 (9) cell lysate.

Immunofluorescence analysis

Figure 3: Immunofluorescence analysis of 3T3-L1 cells using CCT2 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.

Flow cytometric