CCND1 Primary Antibody

Item Information
Catalog # Size/Concentration Price
31985 100ug $358.00
Specification
AliasesBCL1; PRAD1; U21B31; D11S287E
Clone#2A5C9
Entrez GeneID595
FormulationPurified antibody in PBS with 0.05% sodium azide
HostMouse
IsotypeMouse IgG1
ImmunogenPurified recombinant fragment of human CCND1 (AA: 1-295) expressed in E. Coli.
MW33.7kDa
Application
ELISA1/10000
FCM (Flow Cytometry)1/200-1/400
WB (Western Blot)1/500 - 1/2000
Sequence
AA: 1-295
Catalog
31985
$358.00
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Images

ELISA

Cell Culture Products
Figure 1: Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng)

WESTERN BLOT

Cell Culture Products
Figure 2: Western blot analysis using CCND1 mAb against human CCND1 (AA: 1-295) recombinant protein. (Expected MW is 36.6 kDa)

WESTERN BLOT

Cell Culture Products
Figure 3: Western blot analysis using CCND1 mAb against HEK293-6e (1) and CCND1 (AA: 1-295)-hIgGFc transfected HEK293 (2) cell lysate.

FLOW CYTOMETRY

Cell Culture Products
Figure 4: Flow cytometric analysis of Hela cells using CCND1 mouse mAb (green) and negative control (red).

WESTERN BLOT

Cell Culture Products
Figure 5: Western blot analysis using CCND1 mouse mAb against LNCAP (1), and NIH/3T3 (2) cell lysate.
Product Overview
Description

The protein encoded by this gene belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance throughout the cell cycle. Cyclins function as regulators of CDK kinases. Different cyclins exhibit distinct expression and degradation patterns which contribute to the temporal coordination of each mitotic event. This cyclin forms a complex with and functions as a regulatory subunit of CDK4 or CDK6, whose activity is required for cell cycle G1/S transition. This protein has been shown to interact with tumor suppressor protein Rb and the expression of this gene is regulated positively by Rb. Mutations, amplification and overexpression of this gene, which alters cell cycle progression, are observed frequently in a variety of human cancers.

References (references)
References (references) 1.Cancer Med. 2019 Aug;8(9):4100-4109. 2.Sci Rep. 2018 May 1;8(1):6824.

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