CCNA2 Primary Antibody
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Description
The protein encoded by this gene belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance through the cell cycle. Cyclins function as regulators of CDK kinases. Different cyclins exhibit distinct expression and degradation patterns which contribute to the temporal coordination of each mitotic event. In contrast to cyclin A1, which is present only in germ cells, this cyclin is expressed in all tissues tested. This cyclin binds and activates CDC2 or CDK2 kinases, and thus promotes both cell cycle G1/S and G2/M transitions.
Product Overview
Formulation
Purified antibody in PBS with 0.05% sodium azide.
Storage
4°C; -20°C for long term storage
Product Image
Western Blot

Figure 1: Western blot analysis using CCNA2 mAb against human CCNA2 (AA: 105-233) recombinant protein. (Expected MW is 40.8 kDa)
Western Blot

Figure 2: Western blot analysis using CCNA2 mAb against HEK293 (1) and CCNA2 (AA: 105-233)-hIgGFc transfected HEK293 (2) cell lysate.
Western Blot

Figure 3: Western blot analysis using CCNA2 mouse mAb against Hela (1), HEK293 (2), Jurkat (3), K562 (4), SK-Br-3 (5), NIH/3T3 (6) cell lysate.
Flow cytometric

Figure 4: Flow cytometric analysis of A431 cells using CCNA2 mouse mAb (green) and negative control (red).
Immunohistochemical analysis

Figure 5: Immunohistochemical analysis of paraffin-embedded cervical cancer tissues using CCNA2 mouse mAb with DAB staining.
Immunohistochemical analysis

Figure 6: Immunohistochemical analysis of paraffin-embedded bladder cancer tissues using CCNA2 mouse mAb with DAB staining.
Elisa

Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);
For Research Use Only. Not for use in diagnostic procedures.