CCL2 Primary Antibody

Item Information

Catalog #

Size

Price

Description

This gene is one of several cytokine genes clustered on the q-arm of chromosome 17. Cytokines are a family of secreted proteins involved in immunoregulatory and inflammatory processes. The protein encoded by this gene is structurally related to the CXC subfamily of cytokines. Members of this subfamily are characterized by two cysteines separated by a single amino acid. This cytokine displays chemotactic activity for monocytes and basophils but not for neutrophils or eosinophils. It has been implicated in the pathogenesis of diseases characterized by monocytic infiltrates, like psoriasis, rheumatoid arthritis and atherosclerosis. It binds to chemokine receptors CCR2 and CCR4.

Product Overview

Entrez GenelD

6347

Aliases

HC11; MCAF; MCP1; MCP-1; SCYA2; GDCF-2; SMC-CF; HSMCR30

Clone#

2D8

Host / Isotype

Mouse / IgG1

Species Reactivity

Human, Mouse, Monkey

Immunogen

Purified recombinant fragment of human CCL2 expressed in E. Coli.

Formulation

Purified antibody in PBS with 0.05% sodium azide

Storage

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.

Product Applications

WB (Western Blot)

1/500 - 1/2000

IHC_P(Immunohistochemistry)

1/200 - 1/1000

ICC (Immunocytochemistry)

1/200 - 1/1000

FCM (Flow Cytometry)

1/200 - 1/400

ELISA

1/10000

References

J Cereb Blood Flow Metab. 2010 Mar;30(3):459-73.
Prostate. 2010 Mar 1;70(4):433-42.

Product Image

Western Blot

Figure 1: Western blot analysis using CCL2 mAb against human CCL2 (AA: 1-99) recombinant protein. (Expected MW is 36.5 kDa)

Western Blot

Figure 2: Western blot analysis using CCL2 mouse mAb against A549 (1), HeLa (2), Raw264.7 (3), L1210 (4), C6 (5), and COS-7 (6)cell lysate.

Immunohistochemical analysis

Figure 3: Immunohistochemical analysis of paraffin-embedded liver cancer tissues using CCL2 mouse mAb with DAB staining.

Immunofluorescence analysis

Figure 4: Immunofluorescence analysis of HepG2 cells. Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.

Immunofluorescence analysis

Figure 5: Immunofluorescence analysis of HepG2 cells using CCL2 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.

Flow cytometric