CBX5 Primary Antibody

Item Information

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Size

Price

Description

This gene encodes a highly conserved nonhistone protein, which is a member of the heterochromatin protein family. The protein is enriched in the heterochromatin and associated with centromeres. The protein has a single N-terminal chromodomain which can bind to histone proteins via methylated lysine residues, and a C-terminal chromo shadow-domain (CSD) which is responsible for the homodimerization and interaction with a number of chromatin-associated nonhistone proteins. The encoded product is involved in the formation of functional kinetochore through interaction with essential kinetochore proteins. The gene has a pseudogene located on chromosome 3. Multiple alternatively spliced variants, encoding the same protein, have been identified.

Product Overview

Entrez GenelD

23468

Aliases

HP1; HP1A; HEL25

Clone#

1B10B2

Host / Isotype

Mouse / IgG1

Species Reactivity

Human, Mouse

Immunogen

Purified recombinant fragment of human CBX5 (AA: 1-191) expressed in E. Coli.

Formulation

Purified antibody in PBS with 0.05% sodium azide

Storage

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.

Product Applications

WB (Western Blot)

1/500 - 1/2000

IHC_P(Immunohistochemistry)

1/200 - 1/1000

ICC (Immunocytochemistry)

1/200 - 1/1000

ELISA

1/10000

References

1.Mol Carcinog. 2011 Aug;50(8):601-13.
2.J Mol Biol. 2013 Jan 9;425(1):54-70.

Product Image

Elisa

Figure 1: Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);

Western Blot

Figure 2:Western blot analysis using CBX5 mAb against human CBX5 (AA: 1-191) recombinant protein. (Expected MW is 48.2 kDa)

Western Blot

Figure 3:Western blot analysis using CBX5 mAb against HEK293 (1) and CBX5 (AA: 1-191)-hIgGFc transfected HEK293 (2) cell lysate.

Western Blot

Figure 4:Western blot analysis using CBX5 mouse mAb against Hela (1), NIH/3T3 (2), K562 (3), MCF-7 (4), Jurkat (5), and A431 (6) cell lysate.

Immunofluorescence analysis

Figure 5:Immunofluorescence analysis of Hela cells using CBX5 mouse mAb. Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin.

Immunofluorescence analysis

Figure 6:Immunofluorescence analysis of Hela cells using CBX5 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)

Immunohistochemical analysis