CBX5 Primary Antibody
|Aliases||HP1; HP1A; HEL25|
|Formulation||Purified antibody in PBS with 0.05% sodium azide|
|Immunogen||Purified recombinant fragment of human CBX5 (AA: 1-191) expressed in E. Coli.|
|Shipping Information||This product will ship in a box containing blue ice at a temperature of 4°C. Learn More|
|Species Reactivity||Human, Mouse|
|ICC (Immunocytochemistry)||1/200 - 1/1000|
|IHC_P(Immunohistochemistry)||1/200 - 1/1000|
|WB (Western Blot)||1/500 - 1/2000|
Figure 1: Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);
Figure 2:Western blot analysis using CBX5 mAb against human CBX5 (AA: 1-191) recombinant protein. (Expected MW is 48.2 kDa)
Figure 3:Western blot analysis using CBX5 mAb against HEK293 (1) and CBX5 (AA: 1-191)-hIgGFc transfected HEK293 (2) cell lysate.
Figure 4:Western blot analysis using CBX5 mouse mAb against Hela (1), NIH/3T3 (2), K562 (3), MCF-7 (4), Jurkat (5), and A431 (6) cell lysate.
Figure 5:Immunofluorescence analysis of Hela cells using CBX5 mouse mAb. Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin.
Figure 6:Immunofluorescence analysis of Hela cells using CBX5 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)
Figure 7:Immunohistochemical analysis of paraffin-embedded esophageal cancer tissues using CBX5 mouse mAb with DAB staining.
Figure 8:Immunohistochemical analysis of paraffin-embedded esophagus tissues using CBX5 mouse mAb with DAB staining.
|Description||This gene encodes a highly conserved nonhistone protein, which is a member of the heterochromatin protein family. The protein is enriched in the heterochromatin and associated with centromeres. The protein has a single N-terminal chromodomain which can bind to histone proteins via methylated lysine residues, and a C-terminal chromo shadow-domain (CSD) which is responsible for the homodimerization and interaction with a number of chromatin-associated nonhistone proteins. The encoded product is involved in the formation of functional kinetochore through interaction with essential kinetochore proteins. The gene has a pseudogene located on chromosome 3. Multiple alternatively spliced variants, encoding the same protein, have been identified. |
|References (references)||1.Mol Carcinog. 2011 Aug;50(8):601-13. |
2.J Mol Biol. 2013 Jan 9;425(1):54-70.