CBX1 Primary Antibody

Item Information
Catalog #Size/ConcentrationPrice
Specification
AliasesCBX; M31; MOD1; p25beta; HP1-BETA; HP1Hsbeta; HP1Hs-beta
ProductOrderC
Clone#4D7B8
Entrez GenelD10951
FormulationPurified antibody in PBS with 0.05% sodium azide
HostMouse
IsotypeIgG1
ImmunogenPurified recombinant fragment of human CBX1 (AA: 1-185) expressed in E. Coli.
MW21.4kDa
Shipping InformationThis product will ship in a box containing blue ice at a temperature of 4°C. Learn More
Species ReactivityHuman
Application
ELISA1/10000
ICC (Immunocytochemistry)1/200 - 1/1000
IHC_P(Immunohistochemistry)1/200 - 1/1000
FCM (Flow Cytometry)1/200 - 1/400
WB (Western Blot)1/500 - 1/2000
Sequence
FULL(1-185)
Catalog#: 30802
Inquire
Contact us to order
Images
Elisa
Figure 1: Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);
Figure 1: Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);
Western Blot
Figure 2:Western blot analysis using CBX1 mAb against human CBX1 (AA: 1-185) recombinant protein. (Expected MW is 47.3 kDa)
Figure 2:Western blot analysis using CBX1 mAb against human CBX1 (AA: 1-185) recombinant protein. (Expected MW is 47.3 kDa)
Western Blot
Figure 3:Western blot analysis using CBX1 mAb against HEK293 (1) and CBX1 (AA: 1-185)-hIgGFc transfected HEK293 (2) cell lysate.
Figure 3:Western blot analysis using CBX1 mAb against HEK293 (1) and CBX1 (AA: 1-185)-hIgGFc transfected HEK293 (2) cell lysate.
Immunofluorescence analysis
Figure 4:Immunofluorescence analysis of Hela cells using CBX1 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)
Figure 4:Immunofluorescence analysis of Hela cells using CBX1 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)
Flow cytometric
Figure 5:Flow cytometric analysis of Hela cells using CBX1 mouse mAb (green) and negative control (red).
Figure 5:Flow cytometric analysis of Hela cells using CBX1 mouse mAb (green) and negative control (red).
Immunohistochemical analysis
Figure 6:Immunohistochemical analysis of paraffin-embedded cervical cancer tissues using CBX1 mouse mAb with DAB staining.
Figure 6:Immunohistochemical analysis of paraffin-embedded cervical cancer tissues using CBX1 mouse mAb with DAB staining.
Immunohistochemical analysis
Figure 7:Immunohistochemical analysis of paraffin-embedded ovarian cancer tissues using CBX1 mouse mAb with DAB staining.
Figure 7:Immunohistochemical analysis of paraffin-embedded ovarian cancer tissues using CBX1 mouse mAb with DAB staining.
Immunohistochemical analysis
Figure 8:Immunohistochemical analysis of paraffin-embedded colon cancer tissues using CBX1 mouse mAb with DAB staining.
Figure 8:Immunohistochemical analysis of paraffin-embedded colon cancer tissues using CBX1 mouse mAb with DAB staining.
Product Overview
DescriptionThis gene encodes a highly conserved nonhistone protein, which is a member of the heterochromatin protein family . The protein is enriched in the heterochromatin and associated with centromeres. The protein has a single N-terminal chromodomain which can bind to histone proteins via methylated lysine residues, and a C-terminal chromo shadow-domain (CSD) which is responsible for the homodimerization and interaction with a number of chromatin-associated nonhistone proteins. The protein may play an important role in the epigenetic control of chromatin structure and gene expression. Several related pseudogenes are located on chromosomes 1, 3, and X. Multiple alternatively spliced variants, encoding the same protein, have been identified.
References (references)
References (references)1.Endocr Relat Cancer. 2010 May 18;17(2):455-67.
2.Am J Pathol. 2011 Feb;178(2):672-8.