CASP8 Primary Antibody

Item Information
Catalog #Size/ConcentrationPrice
Specification
AliasesCAP4; MACH; MCH5; FLICE; ALPS2B; Casp-8; FLJ17672; MGC78473
ProductOrderC
Clone#1H11
Entrez GenelD841
FormulationAscitic fluid containing 0.03% sodium azide.
HostMouse
IsotypeIgG1
ImmunogenPurified recombinant fragment of human CASP8 expressed in E. Coli.
MW26kDa
Shipping InformationThis product will ship in a box containing blue ice at a temperature of 4°C. Learn More
Species ReactivityHuman, Mouse, Monkey, Rat
Application
ELISA1/10000
IHC_P(Immunohistochemistry)1/200 - 1/1000
FCM (Flow Cytometry)1/200 - 1/400
WB (Western Blot)1/500 - 1/2000
Sequence
302-464
Catalog#: 30279
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Images
Western Blot
Figure 1: Western blot analysis using CASP8 mouse mAb against Hela (1), Jurkat (2), THP-1 (3), NIH/3T3 (4), Cos7 (5) and PC-12 (6) cell lysate.
Figure 1: Western blot analysis using CASP8 mouse mAb against Hela (1), Jurkat (2), THP-1 (3), NIH/3T3 (4), Cos7 (5) and PC-12 (6) cell lysate.
Immunohistochemical analysis
Figure 2: Immunohistochemical analysis of paraffin-embedded colon cancer tissues using CASP8 mouse mAb with DAB staining.
Figure 2: Immunohistochemical analysis of paraffin-embedded colon cancer tissues using CASP8 mouse mAb with DAB staining.
Immunohistochemical analysis
Figure 3: Immunohistochemical analysis of paraffin-embedded liver cancer tissues using CASP8 mouse mAb with DAB staining.
Figure 3: Immunohistochemical analysis of paraffin-embedded liver cancer tissues using CASP8 mouse mAb with DAB staining.
Flow cytometric
Figure 4: Flow cytometric analysis of NIH/3T3 cells using CASP8 mouse mAb (blue) and negative control (red).
Figure 4: Flow cytometric analysis of NIH/3T3 cells using CASP8 mouse mAb (blue) and negative control (red).
Elisa
Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);
Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);
Product Overview
Description

This gene encodes a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes composed of a prodomain, a large protease subunit, and a small protease subunit. Activation of caspases requires proteolytic processing at conserved internal aspartic residues to generate a heterodimeric enzyme consisting of the large and small subunits. This protein is involved in the programmed cell death induced by Fas and various apoptotic stimuli. The N-terminal FADD-like death effector domain of this protein suggests that it may interact with Fas-interacting protein FADD. This protein was detected in the insoluble fraction of the affected brain region from Huntington disease patients but not in those from normal controls, which implicated the role in neurodegenerative diseases. Many alternatively spliced transcript variants encoding different isoforms have been described, although not all variants have had their full-length sequences determined.

References (references)
References (references)1. Cancer Lett. 2009 Aug 28;281(2):128-33.
2. Cell Res. 2009 Mar;19(3):358-69.