CARM1 Primary Antibody

Item Information

Catalog #

Size

Price

Description

Protein arginine N-methyltransferases, such as CARM1, catalyze the transfer of a methyl group from S-adenosyl-L-methionine to the side chain nitrogens of arginine residues within proteins to form methylated arginine derivatives and S-adenosyl-L-homocysteine. Protein arginine methylation has been implicated in signal transduction, metabolism of nascent pre-RNA, and transcriptional activation (Frankel et al. 2002 (PubMed 11724789). Tissue specificity: Overexpressed in prostate adenocarcinomas and high-grade prostatic intraepithelial neoplasia.

Product Overview

Entrez GenelD

10498

Aliases

PRMT4; CARM1

Clone#

3H2

Host / Isotype

Mouse / IgG1

Species Reactivity

Human, Monkey, Rat

Immunogen

Purified recombinant fragment of human CARM1 expressed in E. Coli.

Formulation

Ascitic fluid containing 0.03% sodium azide.

Storage

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.

Product Applications

WB (Western Blot)

1/500 - 1/2000

IHC_P(Immunohistochemistry)

1/200 - 1/1000

ICC (Immunocytochemistry)

1/200 - 1/1000

FCM (Flow Cytometry)

1/200 - 1/400

ELISA

1/10000

References

1. FASEB J. 2008 Sep;22(9):3337-47.
2. Nucleic Acids Res. 2008 Jun;36(10):3202-13.

Product Image

Western Blot

Figure 1: Western blot analysis using CARM1 mouse mAb against MCF-7 (1), Hela (2), NIH/3T3 (3), HL-60 (4), LNcap (5), Jurkat (6), PC-3 (7), Cos7 (8), and PC-12 (9) cell lysate.

Immunohistochemical analysis

Figure 2: Immunohistochemical analysis of paraffin-embedded breast cancer tissues (left) and ovarian cancer tissues (right) using CARM1 mouse mAb with DAB staining.

Immunofluorescence analysis

Figure 3: Immunofluorescence analysis of Hela cells using CRAM1 mouse mAb (green). Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.

Flow cytometric