CALR Primary Antibody

Item Information
Catalog # Size/Concentration Price
31799 100ug $341.00
Specification
AliasesRO; CRT; SSA; cC1qR; HEL-S-99n
Clone#7B3D7
Entrez GeneID811
FormulationPurified antibody in PBS with 0.05% sodium azide
HostMouse
IsotypeMouse IgG1
ImmunogenPurified recombinant fragment of human CALR (AA: 18-417) expressed in E. Coli.
MW48.1kDa
Application
ELISA1/10000
IHC_P (Immunohistochemistry) 1/200 - 1/1000
FCM (Flow Cytometry)1/200 - 1/400
WB (Western Blot)1/500 - 1/2000
Sequence
18-417
Catalog
31799
$341.00
-
+

Contact us to order

Images

Elisa

Cell Culture Products

Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)

Western Blot

Cell Culture Products

Figure 2:Western blot analysis using CALR mAb against human CALR (AA: 18-417) recombinant protein. (Expected MW is 49.4 kDa)

Western Blot

Cell Culture Products

Figure 3:Western blot analysis using CALR mAb against HEK293 (1) and CALR (AA: 18-417)-hIgGFc transfected HEK293 (2) cell lysate.

Western Blot

Cell Culture Products

Figure 4:Western blot analysis using CALR mouse mAb against Hela (1), MCF-7 (2), NIH/3T3 (3), HepG2 (4), Jurkat (5), Y-79 (6), and C6 (7) cell lysate.

Flow Cytometric

Cell Culture Products

Figure 5:Flow cytometric analysis of Hela cells using CALR mouse mAb (green) and negative control (red).

Immunohistochemical Analysis

Cell Culture Products

Figure 6:Immunohistochemical analysis of paraffin-embedded cervical cancer tissues using CALR mouse mAb with DAB staining.

Immunohistochemical Analysis

Cell Culture Products

Figure 7:Immunohistochemical analysis of paraffin-embedded bladder cancer tissues using CALR mouse mAb with DAB staining.

Product Overview
Description

Calreticulin is a multifunctional protein that acts as a major Ca(2+)-binding (storage) protein in the lumen of the endoplasmic reticulum. It is also found in the nucleus, suggesting that it may have a role in transcription regulation. Calreticulin binds to the synthetic peptide KLGFFKR, which is almost identical to an amino acid sequence in the DNA-binding domain of the superfamily of nuclear receptors. Calreticulin binds to antibodies in certain sera of systemic lupus and Sjogren patients which contain anti-Ro/SSA antibodies, it is highly conserved among species, and it is located in the endoplasmic and sarcoplasmic reticulum where it may bind calcium. The amino terminus of calreticulin interacts with the DNA-binding domain of the glucocorticoid receptor and prevents the receptor from binding to its specific glucocorticoid response element. Calreticulin can inhibit the binding of androgen receptor to its hormone-responsive DNA element and can inhibit androgen receptor and retinoic acid receptor transcriptional activities in vivo, as well as retinoic acid-induced neuronal differentiation. Thus, calreticulin can act as an important modulator of the regulation of gene transcription by nuclear hormone receptors. Systemic lupus erythematosus is associated with increased autoantibody titers against calreticulin but calreticulin is not a Ro/SS-A antigen. Earlier papers referred to calreticulin as an Ro/SS-A antigen but this was later disproven. Increased autoantibody titer against human calreticulin is found in infants with complete congenital heart block of both the IgG and IgM classes.

References (references)
References (references) 1.Endocrinology. 2017 Nov 1;158(11):3874-3889. 2.Haematologica. 2017 Oct;102(10):e394-e396.

Please view FAQs here