CA9 Primary Antibody
Item Information
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Description
CA IX is a transmembrane protein and the only tumor-associated carbonic anhydrase isoenzyme known. It is expressed in all clear-cell renal cell carcinoma, but is not detected in normal kidney or most other normal tissues. It may be involved in cell proliferation and transformation. Reversible hydration of carbon dioxide. Participates in pH regulation. May be involved in the control of cell proliferation and transformation. Appears to be a novel specific biomarker for a cervical neoplasia. Tissue specificity: Expressed primarily in carcinoma cells lines. Expression is restricted to very few normal tissues and the most abundant expression is found in the epithelial cells of gastric mucosa.
Product Overview
Entrez GenelD
768
Aliases
MN; CAIX; CA9
Clone#
2D3
Host / Isotype
Mouse / IgG1
Species Reactivity
Human
Immunogen
Purified recombinant fragment of human CA9 expressed in E. Coli.
Formulation
Ascitic fluid containing 0.03% sodium azide.
Storage
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Product Applications
WB (Western Blot)
1/500 - 1/2000
IHC_P(Immunohistochemistry)
1/200 - 1/1000
FCM (Flow Cytometry)
1/200 - 1/400
ELISA
1/10000
References
1. Br J Cancer. 2008 Sep 2;99(5):727-33.
2. Pathol Res Pract. 2009;205(1):1-9.
2. Pathol Res Pract. 2009;205(1):1-9.
Product Image
Western Blot
Figure 1: Western blot analysis using CA9 mouse mAb against Hela (1) and A549 (2) cell lysate.
Immunohistochemical analysis
Figure 2: Immunohistochemical analysis of paraffin-embedded stomach tissues (left) and colon tissues (right) using CA9 mouse mAb with DAB staining.
Flow cytometric
Figure 3: Flow cytometric analysis of NTERA-2 cells using CA9 mouse mAb (green) and negative control (purple).
For Research Use Only. Not for use in diagnostic procedures.