c-Jun Primary Antibody

Item Information

Catalog #

Size

Price

Description

This gene is the putative transforming gene of avian sarcoma virus 17. It encodes a protein which is highly similar to the viral protein, and which interacts directly with specific target DNA sequences to regulate gene expression. This gene is intronless and is mapped to 1p32-p31, a chromosomal region involved in both translocations and deletions in human malignancies.

Product Overview

Entrez GenelD

3725

Aliases

AP1; AP-1; c-Jun; Jun

Clone#

5B1

Host / Isotype

Mouse / IgG1

Species Reactivity

Human, Mouse, Monkey

Immunogen

Purified recombinant fragment of human c-Jun expressed in E. Coli.

Formulation

Ascitic fluid containing 0.03% sodium azide.

Storage

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.

Product Applications

WB (Western Blot)

1/500 - 1/2000

IHC_P(Immunohistochemistry)

1/200 - 1/1000

ICC (Immunocytochemistry)

1/200 - 1/1000

FCM (Flow Cytometry)

1/200 - 1/400

ELISA

1/10000

References

1. J Biol Chem. 2010 Mar 12;285(11):8218-26.
2. Mol Cancer. 2010 May 19;9:111.

Product Image

Western Blot

Figure 1: Western blot analysis using c-Jun mAb against human c-Jun (AA: 199-331) recombinant protein.

Western Blot

Figure 2: Western blot analysis using c-Jun mouse mAb against NIH/3T3 (1) and Cos7 (2) cell lysate.

Immunohistochemical analysis

Figure 3: Immunohistochemical analysis of paraffin-embedded human intima canncer tissues using c-Jun mouse mAb with DAB staining.

Immunohistochemical analysis

Figure 4: Immunohistochemical analysis of paraffin-embedded human rectum cancer tissues using c-Jun mouse mAb with DAB staining.

Immunofluorescence analysis

Figure 5: Immunofluorescence analysis of PC-2 cells using c-Jun mouse mAb (green). Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.

Flow cytometric