BTN2A2 Primary Antibody
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Description
Butyrophilin is the major protein associated with fat droplets in the milk. This gene is a member of the BTN2 subfamily of genes, which encode proteins belonging to the butyrophilin protein family. The gene is located in a cluster on chromosome 6, consisting of seven genes belonging to the expanding B7/butyrophilin-like group, a subset of the immunoglobulin gene superfamily. The encoded protein is a type I receptor glycoprotein involved in lipid, fatty-acid and sterol metabolism. Several alternatively spliced transcript variants encoding different isoforms have been found for this gene.
Product Overview
Formulation
Purified antibody in PBS with 0.05% sodium azide
Storage
4°C; -20°C for long term storage
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Elisa
Figure 1: Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
Western Blot
Figure 2:Western blot analysis using BTN2A2 mAb against human BTN2A2 (AA: extra 57-237) recombinant protein. (Expected MW is 46.4 kDa)
Western Blot
Figure 3:Western blot analysis using BTN2A2 mAb against HEK293 (1) and BTN2A2 (AA: extra 57-237)-hIgGFc transfected HEK293 (2) cell lysate.
Western Blot
Figure 4:Western blot analysis using BTN2A2 mouse mAb against K562 (1) cell lysate.
Flow cytometric
Figure 5:Flow cytometric analysis of Hela cells using BTN2A2 mouse mAb (green) and negative control (red).
Immunofluorescence analysis
Figure 6:Immunofluorescence analysis of Hela cells using BTN2A2 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)
For Research Use Only. Not for use in diagnostic procedures.