BRCA1 Primary Antibody
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Description
This gene encodes a nuclear phosphoprotein that plays a role in maintaining genomic stability, and it also acts as a tumor suppressor. The encoded protein combines with other tumor suppressors, DNA damage sensors, and signal transducers to form a large multi-subunit protein complex known as the BRCA1-associated genome surveillance complex (BASC). This gene product associates with RNA polymerase II, and through the C-terminal domain, also interacts with histone deacetylase complexes. This protein thus plays a role in transcription, DNA repair of double-stranded breaks, and recombination. Mutations in this gene are responsible for approximately 40% of inherited breast cancers and more than 80% of inherited breast and ovarian cancers. Alternative splicing plays a role in modulating the subcellular localization and physiological function of this gene. Many alternatively spliced transcript variants, some of which are disease-associated mutations, have been described for this gene, but the full-length natures of only some of these variants has been described. A related pseudogene, which is also located on chromosome 17, has been identified.
Product Overview
Formulation
Purified antibody in PBS with 0.05% sodium azide
Storage
4°C; -20°C for long term storage
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Western Blot

Figure 1: Western blot analysis using BRCA1 mAb against human BRCA1 recombinant protein. (Expected MW is 37.5 kDa)
Western Blot

Figure 2: Western blot analysis using BRCA1 mAb against HEK293 (1) and BRCA1 (AA: 229-335)-hIgGFc transfected HEK293 (2) cell lysate.
Immunohistochemical analysis

Figure 3: Immunohistochemical analysis of paraffin-embedded esophagus cancer tissues using BRCA1 mouse mAb with DAB staining.
Immunohistochemical analysis

Figure 4: Immunohistochemical analysis of paraffin-embedded esophagus tissues using BRCA1 mouse mAb with DAB staining.
Elisa

Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);
For Research Use Only. Not for use in diagnostic procedures.