BRCA1 Primary Antibody

Item Information
Catalog #Size/ConcentrationPrice
Specification
AliasesIRIS; PSCP; BRCAI; BRCC1; PNCA4; RNF53; BROVCA1; PPP1R53
ProductOrderB
Clone#6C6D2
Entrez GenelD672
FormulationPurified antibody in PBS with 0.05% sodium azide
HostMouse
IsotypeIgG1
ImmunogenPurified recombinant fragment of human BRCA1 (AA: 229-335) expressed in E. Coli.
MW207.7kDa
Shipping InformationThis product will ship in a box containing blue ice at a temperature of 4°C. Learn More
Species ReactivityHuman
Application
ELISA1/10000
IHC_P(Immunohistochemistry)1/200 - 1/1000
WB (Western Blot)1/500 - 1/2000
Sequence
229-335
Catalog#: 30509
Inquire
Contact us to order
1-866-339-0871
510-740-3625
info@promab.com
Images
Western Blot
Figure 1: Western blot analysis using BRCA1 mAb against human BRCA1 recombinant protein. (Expected MW is 37.5 kDa)
Figure 1: Western blot analysis using BRCA1 mAb against human BRCA1 recombinant protein. (Expected MW is 37.5 kDa)
Western Blot
Figure 2: Western blot analysis using BRCA1 mAb against HEK293 (1) and BRCA1 (AA: 229-335)-hIgGFc transfected HEK293 (2) cell lysate.
Figure 2: Western blot analysis using BRCA1 mAb against HEK293 (1) and BRCA1 (AA: 229-335)-hIgGFc transfected HEK293 (2) cell lysate.
Immunohistochemical analysis
Figure 3: Immunohistochemical analysis of paraffin-embedded esophagus cancer tissues using BRCA1 mouse mAb with DAB staining.
Figure 3: Immunohistochemical analysis of paraffin-embedded esophagus cancer tissues using BRCA1 mouse mAb with DAB staining.
Immunohistochemical analysis
Figure 4: Immunohistochemical analysis of paraffin-embedded esophagus tissues using BRCA1 mouse mAb with DAB staining.
Figure 4: Immunohistochemical analysis of paraffin-embedded esophagus tissues using BRCA1 mouse mAb with DAB staining.
Elisa
Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);
Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);
Product Overview
DescriptionThis gene encodes a nuclear phosphoprotein that plays a role in maintaining genomic stability, and it also acts as a tumor suppressor. The encoded protein combines with other tumor suppressors, DNA damage sensors, and signal transducers to form a large multi-subunit protein complex known as the BRCA1-associated genome surveillance complex (BASC). This gene product associates with RNA polymerase II, and through the C-terminal domain, also interacts with histone deacetylase complexes. This protein thus plays a role in transcription, DNA repair of double-stranded breaks, and recombination. Mutations in this gene are responsible for approximately 40% of inherited breast cancers and more than 80% of inherited breast and ovarian cancers. Alternative splicing plays a role in modulating the subcellular localization and physiological function of this gene. Many alternatively spliced transcript variants, some of which are disease-associated mutations, have been described for this gene, but the full-length natures of only some of these variants has been described. A related pseudogene, which is also located on chromosome 17, has been identified.


References (references)
References (references)1. Cancer Res. 2013 Jan 15;73(2):706-15.
2. J Biol Chem. 2012 Nov 23;287(48):40618-28.
Please view FAQs here