BRAF Primary Antibody

Item Information
Catalog #Size/ConcentrationPrice
Specification
AliasesBRAF1; RAFB1; B-RAF1; FLJ95109
ProductOrderB
Clone#1H12
Entrez GenelD673
FormulationAscitic fluid containing 0.03% sodium azide.
HostMouse
IsotypeIgG1
ImmunogenPurified recombinant fragment of human BRAF expressed in E. Coli.
MW87kDa
Shipping InformationThis product will ship in a box containing blue ice at a temperature of 4°C. Learn More
Species ReactivityHuman, Mouse
Application
ELISA1/10000
ICC (Immunocytochemistry)1/200 - 1/1000
IHC_P(Immunohistochemistry)1/200 - 1/1000
WB (Western Blot)1/500 - 1/2000
Sequence
285-401
Catalog#: 20323
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Images
Western Blot
Figure 1: Western blot analysis using BRAF mouse mAb against Hela (1), HL60 (2), HepG2 (3) and NIH/3T3 (4) cell lysate.
Figure 1: Western blot analysis using BRAF mouse mAb against Hela (1), HL60 (2), HepG2 (3) and NIH/3T3 (4) cell lysate.
Immunohistochemical analysis
Figure 2: Immunohistochemical analysis of paraffin-embedded human testis tissues using BRAF mouse mAb.
Figure 2: Immunohistochemical analysis of paraffin-embedded human testis tissues using BRAF mouse mAb.
Immunofluorescence analysis
Figure 3: Confocal Immunofluorescence analysis of MCF-7 (left) and HepG2 (right) cells using BRAF mouse mAb (green). Red: Actin filaments have been labeled with DY-554 phalloidin. Blue: DRAQ5 fluorescent DNA dye
Figure 3: Confocal Immunofluorescence analysis of MCF-7 (left) and HepG2 (right) cells using BRAF mouse mAb (green). Red: Actin filaments have been labeled with DY-554 phalloidin. Blue: DRAQ5 fluorescent DNA dye
Product Overview
Description

BRAF: v-raf murine sarcoma viral oncogene homolog B1, also known as BRAF1; RAFB1; B-RAF1; FLJ95109. Entrez Protein NP_004324. It is the main effectors recruited by GTP-bound Ras to activate the MEK-MAP kinase pathway. B-Raf contains three consensus Akt phosphorylationsites (Ser364, Ser428, and Thr439). B-Raf is a key regulatory molecule of the mitogen-activated protein kinase kinase (MEK), it has a long amino-terminal region,the region is essential for homo-dimerization of B-Raf and hetero-dimerization of B-Raf and c-Raf at the plasma membrane, followed by phosphorylation of Thr118 in the amino-terminal B-Raf-specific region. Notably, in calcium ionophore-stimulated HeLa cells, B-Raf could propagate signals to MEK under the basal level of GTP-Ras. Expression of Raf-B is highly restricted with highestlevels in the cerebrum and testes and defects in braf are involved in a wide range of cancers. The BRAF gene mutation is frequently detected in papillary thyroid carcinoma,melanocytic nevi, primary cutaneous melanomas and colorectal cancers.

References (references)
References (references)1. J Clin Oncol. 2008 Dec 10;26(35):5705-12.
2. Endocr Relat Cancer. 2006 Mar;13(1):257-69.