BRAF Primary Antibody
BRAF: v-raf murine sarcoma viral oncogene homolog B1, also known as BRAF1; RAFB1; B-RAF1; FLJ95109. Entrez Protein NP_004324. It is the main effectors recruited by GTP-bound Ras to activate the MEK-MAP kinase pathway. B-Raf contains three consensus Akt phosphorylationsites (Ser364, Ser428, and Thr439). B-Raf is a key regulatory molecule of the mitogen-activated protein kinase kinase (MEK), it has a long amino-terminal region,the region is essential for homo-dimerization of B-Raf and hetero-dimerization of B-Raf and c-Raf at the plasma membrane, followed by phosphorylation of Thr118 in the amino-terminal B-Raf-specific region. Notably, in calcium ionophore-stimulated HeLa cells, B-Raf could propagate signals to MEK under the basal level of GTP-Ras. Expression of Raf-B is highly restricted with highestlevels in the cerebrum and testes and defects in braf are involved in a wide range of cancers. The BRAF gene mutation is frequently detected in papillary thyroid carcinoma,melanocytic nevi, primary cutaneous melanomas and colorectal cancers.
2. Endocr Relat Cancer. 2006 Mar;13(1):257-69.
![Figure 1: Western blot analysis using BRAF mouse mAb against Hela (1), HL60 (2), HepG2 (3) and NIH/3T3 (4) cell lysate.](https://cdn.shopify.com/s/files/1/0604/2661/7019/products/20323lwb.jpg?v=1666698741)
![Figure 2: Immunohistochemical analysis of paraffin-embedded human testis tissues using BRAF mouse mAb.](https://cdn.shopify.com/s/files/1/0604/2661/7019/products/20323ihc.jpg?v=1666698741)
![Figure 3: Confocal Immunofluorescence analysis of MCF-7 (left) and HepG2 (right) cells using BRAF mouse mAb (green). Red: Actin filaments have been labeled with DY-554 phalloidin. Blue: DRAQ5 fluorescent DNA dye](https://cdn.shopify.com/s/files/1/0604/2661/7019/products/20323icc.jpg?v=1666698741)