BMP7 Primary Antibody

Item Information
Catalog #Size/ConcentrationPrice
Specification
AliasesOP-1
ProductOrderB
Clone#6E5D12
Entrez GenelD655
FormulationPurified antibody in PBS with 0.05% sodium azide
HostMouse
IsotypeIgG1
ImmunogenPurified recombinant fragment of human BMP7 (AA: 239-431) expressed in E. Coli.
MW49.3kDa
Shipping InformationThis product will ship in a box containing blue ice at a temperature of 4°C. Learn More
Species ReactivityHuman, Mouse
Application
ELISA1/10000
IHC_P(Immunohistochemistry)1/200 - 1/1000
FCM (Flow Cytometry)1/200 - 1/400
WB (Western Blot)1/500 - 1/2000
Sequence
239-431
Catalog#: 30875
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Images
Elisa
Figure 1: Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);
Figure 1: Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);
Western Blot
Figure 2:Western blot analysis using BMP7 mAb against human BMP7 (AA: 239-431) recombinant protein. (Expected MW is 47.7 kDa)
Figure 2:Western blot analysis using BMP7 mAb against human BMP7 (AA: 239-431) recombinant protein. (Expected MW is 47.7 kDa)
Western Blot
Figure 3:Western blot analysis using BMP7 mAb against HEK293 (1) and BMP7 (AA: 239-431)-hIgGFc transfected HEK293 (2) cell lysate.
Figure 3:Western blot analysis using BMP7 mAb against HEK293 (1) and BMP7 (AA: 239-431)-hIgGFc transfected HEK293 (2) cell lysate.
Western Blot
Figure 4:Western blot analysis using BMP7 mouse mAb against Raw264.7 (1), A549 (2), Jurkat (3), PC-3 (4), HEK293 (5), Jurkat (6), NIH/3T3 (7), and Hela (8) cell lysate.
Figure 4:Western blot analysis using BMP7 mouse mAb against Raw264.7 (1), A549 (2), Jurkat (3), PC-3 (4), HEK293 (5), Jurkat (6), NIH/3T3 (7), and Hela (8) cell lysate.
Flow cytometric
Figure 5:Flow cytometric analysis of HEK293 cells using BMP7 mouse mAb (green) and negative control (red).
Figure 5:Flow cytometric analysis of HEK293 cells using BMP7 mouse mAb (green) and negative control (red).
Immunohistochemical analysis
Figure 6:Immunohistochemical analysis of paraffin-embedded esophageal cancer tissues using BMP7 mouse mAb with DAB staining.
Figure 6:Immunohistochemical analysis of paraffin-embedded esophageal cancer tissues using BMP7 mouse mAb with DAB staining.
Product Overview
DescriptionThe bone morphogenetic proteins (BMPs) are a family of secreted signaling molecules that can induce ectopic bone growth. Many BMPs are part of the transforming growth factor-beta (TGFB) superfamily. BMPs were originally identified by an ability of demineralized bone extract to induce endochondral osteogenesis in vivo in an extraskeletal site. Based on its expression early in embryogenesis, the BMP encoded by this gene has a proposed role in early development and possible bone inductive activity.
References (references)
References (references)1.Immunol Cell Biol. 2014 May-Jun;92(5):427-35.
2.J Exp Med. 2013 Nov 18;210(12):2597-610.