BMP7 Primary Antibody

Item Information

Catalog #

Size

Price

Description

The bone morphogenetic proteins (BMPs) are a family of secreted signaling molecules that can induce ectopic bone growth. Many BMPs are part of the transforming growth factor-beta (TGFB) superfamily. BMPs were originally identified by an ability of demineralized bone extract to induce endochondral osteogenesis in vivo in an extraskeletal site. Based on its expression early in embryogenesis, the BMP encoded by this gene has a proposed role in early development and possible bone inductive activity.

Product Overview

Entrez GenelD

655

Aliases

OP-1

Clone#

6E5D12

Host / Isotype

Mouse / IgG1

Species Reactivity

Human, Mouse

Immunogen

Purified recombinant fragment of human BMP7 (AA: 239-431) expressed in E. Coli.

Formulation

Purified antibody in PBS with 0.05% sodium azide

Storage

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.

Product Applications

WB (Western Blot)

1/500 - 1/2000

IHC_P(Immunohistochemistry)

1/200 - 1/1000

FCM (Flow Cytometry)

1/200 - 1/400

ELISA

1/10000

References

1.Immunol Cell Biol. 2014 May-Jun;92(5):427-35.
2.J Exp Med. 2013 Nov 18;210(12):2597-610.

Product Image

Elisa

Figure 1: Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);

Western Blot

Figure 2:Western blot analysis using BMP7 mAb against human BMP7 (AA: 239-431) recombinant protein. (Expected MW is 47.7 kDa)

Western Blot

Figure 3:Western blot analysis using BMP7 mAb against HEK293 (1) and BMP7 (AA: 239-431)-hIgGFc transfected HEK293 (2) cell lysate.

Western Blot

Figure 4:Western blot analysis using BMP7 mouse mAb against Raw264.7 (1), A549 (2), Jurkat (3), PC-3 (4), HEK293 (5), Jurkat (6), NIH/3T3 (7), and Hela (8) cell lysate.

Flow cytometric