BDNF Primary Antibody

Item Information

Catalog #

Size

Price

Description

The protein encoded by this gene is a member of the nerve growth factor family. It is induced by cortical neurons, and is necessary for survival of striatal neurons in the brain. Expression of this gene is reduced in both Alzheimer's and Huntington disease patients. This gene may play a role in the regulation of stress response and in the biology of mood disorders. Multiple transcript variants encoding distinct isoforms have been described for this gene.

Product Overview

Entrez GenelD

627

Aliases

ANON2; BULN2

Clone#

3D9C5

Host / Isotype

Mouse / IgG1

Species Reactivity

Human

Immunogen

Purified recombinant fragment of human BDNF (AA: 19-248) expressed in E. Coli.

Formulation

Purified antibody in PBS with 0.05% sodium azide.

Storage

4°C; -20°C for long term storage

Product Applications

WB (Western Blot)

1/500 - 1/2000

ICC (Immunocytochemistry)

1/200 - 1/1000

ELISA

1/10000

References

1. Br J Cancer. 2013 Jan 15;108(1):121-30.
2. PLoS One. 2012;7(8):e42676.

Product Image

Western Blot

Figure 1: Western blot analysis using BDNF mAb against human BDNF (AA: 19-248) recombinant protein. (Expected MW is 51.7 kDa)

Western Blot

Figure 2: Western blot analysis using BDNF mAb against HEK293 (1) and BDNF (AA: 19-248)-hIgGFc transfected HEK293 (2) cell lysate.

Western Blot

Figure 3: Western blot analysis using BDNF mouse mAb against SK-N-SH (1) cell lysate.

Immunofluorescence analysis

Figure 4: Immunofluorescence analysis of Hela cells using BDNF mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Secondary antibody from Fisher (Cat#: 35503)

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