BDNF Primary Antibody

Item Information
Catalog #Size/ConcentrationPrice
Specification
AliasesANON2; BULN2
ProductOrderB
Clone#3D9C5
Entrez GenelD627
FormulationPurified antibody in PBS with 0.05% sodium azide.
HostMouse
IsotypeIgG1
ImmunogenPurified recombinant fragment of human BDNF (AA: 19-248) expressed in E. Coli.
MW27.8kDa
Shipping InformationThis product will ship in a box containing blue ice at a temperature of 4°C. Learn More
Species ReactivityHuman
Application
ELISA1/10000
ICC (Immunocytochemistry)1/200 - 1/1000
WB (Western Blot)1/500 - 1/2000
Sequence
19-248
Catalog#: 30610
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Images
Western Blot
Figure 1: Western blot analysis using BDNF mAb against human BDNF (AA: 19-248) recombinant protein. (Expected MW is 51.7 kDa)
Figure 1: Western blot analysis using BDNF mAb against human BDNF (AA: 19-248) recombinant protein. (Expected MW is 51.7 kDa)
Western Blot
Figure 2: Western blot analysis using BDNF mAb against HEK293 (1) and BDNF (AA: 19-248)-hIgGFc transfected HEK293 (2) cell lysate.
Figure 2: Western blot analysis using BDNF mAb against HEK293 (1) and BDNF (AA: 19-248)-hIgGFc transfected HEK293 (2) cell lysate.
Western Blot
Figure 3: Western blot analysis using BDNF mouse mAb against SK-N-SH (1) cell lysate.
Figure 3: Western blot analysis using BDNF mouse mAb against SK-N-SH (1) cell lysate.
Immunofluorescence analysis
Figure 4: Immunofluorescence analysis of Hela cells using BDNF mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Secondary antibody from Fisher (Cat#: 35503)
Figure 4: Immunofluorescence analysis of Hela cells using BDNF mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Secondary antibody from Fisher (Cat#: 35503)
Elisa
Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);
Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);
Product Overview
DescriptionThe protein encoded by this gene is a member of the nerve growth factor family. It is induced by cortical neurons, and is necessary for survival of striatal neurons in the brain. Expression of this gene is reduced in both Alzheimer's and Huntington disease patients. This gene may play a role in the regulation of stress response and in the biology of mood disorders. Multiple transcript variants encoding distinct isoforms have been described for this gene.
References (references)
References (references)1. Br J Cancer. 2013 Jan 15;108(1):121-30.
2. PLoS One. 2012;7(8):e42676.