BCR Primary Antibody
A reciprocal translocation between chromosomes 22 and 9 produces the Philadelphia chromosome, which is often found in patients with chronic myelogenous leukemia. The chromosome 22 breakpoint for this translocation is located within the BCR gene. The translocation produces a fusion protein which is encoded by sequence from both BCR and ABL, the gene at the chromosome 9 breakpoint. Although the BCR-ABL fusion protein has been extensively studied, the function of the normal BCR gene product is not clear. The protein has serine/threonine kinase activity and is a GTPase-activating protein for p21rac. Two transcript variants encoding different isoforms have been found for this gene.
Purified recombinant fragment of human BCR (AA: 139-280) expressed in E. Coli.
Purified antibody in PBS with 0.05% sodium azide
4°C; -20°C for long term storage
Figure 1: Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
Figure 2:Western blot analysis using BCR mAb against human BCR (AA: 139-280) recombinant protein. (Expected MW is 41.6 kDa)
Figure 3:Western blot analysis using BCR mAb against HEK293 (1) and BCR (AA: 139-280)-hIgGFc transfected HEK293 (2) cell lysate.
Figure 4:Western blot analysis using BCR mouse mAb against Jurkat (1), Hela (2), and Ramos (3) cell lysate.
Figure 5:Flow cytometric analysis of K562 cells using BCR mouse mAb (green) and negative control (red).
For Research Use Only. Not for use in diagnostic procedures.