BCR Primary Antibody
Item Information
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Size
Price
Description
A reciprocal translocation between chromosomes 22 and 9 produces the Philadelphia chromosome, which is often found in patients with chronic myelogenous leukemia. The chromosome 22 breakpoint for this translocation is located within the BCR gene. The translocation produces a fusion protein which is encoded by sequence from both BCR and ABL, the gene at the chromosome 9 breakpoint. Although the BCR-ABL fusion protein has been extensively studied, the function of the normal BCR gene product is not clear. The protein has serine/threonine kinase activity and is a GTPase-activating protein for p21rac. Two transcript variants encoding different isoforms have been found for this gene.
Product Overview
Entrez GenelD
613
Aliases
ALL; CML; PHL; BCR1; D22S11; D22S662
Clone#
1E11G12
Host / Isotype
Mouse / IgG1
Species Reactivity
Human
Immunogen
Purified recombinant fragment of human BCR (AA: 139-280) expressed in E. Coli.
Formulation
Purified antibody in PBS with 0.05% sodium azide
Storage
4°C; -20°C for long term storage
Product Applications
WB (Western Blot)
1/500 - 1/2000
FCM (Flow Cytometry)
1/200 - 1/400
ELISA
1/10000
References
1.Asian Pac J Cancer Prev. 2014;15(22):9961-6.
2.Asian Pac J Cancer Prev. 2014;15(12):4773-80.
2.Asian Pac J Cancer Prev. 2014;15(12):4773-80.
Product Image
Elisa
Figure 1: Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
Western Blot
Figure 2:Western blot analysis using BCR mAb against human BCR (AA: 139-280) recombinant protein. (Expected MW is 41.6 kDa)
Western Blot
Figure 3:Western blot analysis using BCR mAb against HEK293 (1) and BCR (AA: 139-280)-hIgGFc transfected HEK293 (2) cell lysate.
Western Blot
Figure 4:Western blot analysis using BCR mouse mAb against Jurkat (1), Hela (2), and Ramos (3) cell lysate.
Flow cytometric
Figure 5:Flow cytometric analysis of K562 cells using BCR mouse mAb (green) and negative control (red).
For Research Use Only. Not for use in diagnostic procedures.