The protein encoded by this gene is a zinc finger transcription factor and contains an N-terminal POZ domain. This protein acts as a sequence-specific repressor of transcription, and has been shown to modulate the transcription of STAT-dependent IL-4 responses of B cells. This protein can interact with a variety of POZ-containing proteins that function as transcription corepressors. This gene is found to be frequently translocated and hypermutated in diffuse large-cell lymphoma (DLCL), and may be involved in the pathogenesis of DLCL. Alternatively spliced transcript variants encoding different protein isoforms have been found for this gene.
Purified antibody in PBS with 0.05% sodium azide
4℃; -20℃ for long term storage
Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
Figure 2:Western blot analysis using BCL6 mAb against human BCL6 (AA: 507-706) recombinant protein. (Expected MW is 26 kDa)
Figure 3:Western blot analysis using BCL6 mAb against HEK293-6e (1) and BCL6 (AA: 507-706)-hIgGFc transfected HEK293-6e (2) cell lysate.
Figure 4:Western blot analysis using BCL6 mouse mAb against Jurkat (1), K562 (2), Raji (3), Ramos (4) and MOLT-4 (5) cell lysate.
Figure 5:Immunofluorescence analysis of Hela cells using BCL6 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)
Figure 6:Flow cytometric analysis of HL-60 cells using BCL6 mouse mAb (green) and negative control (red).
Figure 7:Immunohistochemical analysis of paraffin-embedded lung cancer tissues using BCL6 mouse mAb with DAB staining.
Figure 8:Immunohistochemical analysis of paraffin-embedded cervical carcinoma tissues using BCL6 mouse mAb with DAB staining.
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