BAK1 Primary Antibody
Item Information
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Size
Price
Description
The protein encoded by this gene belongs to the BCL2 protein family. BCL2 family members form oligomers or heterodimers and act as anti- or pro-apoptotic regulators that are involved in a wide variety of cellular activities. This protein localizes to mitochondria, and functions to induce apoptosis. It interacts with and accelerates the opening of the mitochondrial voltage-dependent anion channel, which leads to a loss in membrane potential and the release of cytochrome c. This protein also interacts with the tumor suppressor P53 after exposure to cell stress.
Product Overview
Entrez GenelD
578
Aliases
BAK; CDN1; BCL2L7; BAK-LIKE
Clone#
2H9H7
Host / Isotype
Mouse / IgG2b
Species Reactivity
Human
Immunogen
Purified recombinant fragment of human BAK1 (AA: 29-187) expressed in E. Coli.
Formulation
Purified antibody in PBS with 0.05% sodium azide
Storage
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Product Applications
WB (Western Blot)
1/500 - 1/2000
IHC_P(Immunohistochemistry)
1/200 - 1/1000
FCM (Flow Cytometry)
1/200 - 1/400
ELISA
1/10000
References
1.Cell Death Differ. 2015 Oct;22(10):1665-75.
2.PLoS Pathog. 2013;9(10):e1003658.
2.PLoS Pathog. 2013;9(10):e1003658.
Product Image
Elisa
Figure 1: Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
Western Blot
Figure 2:Western blot analysis using BAK1 mAb against human BAK1 (AA: 29-187) recombinant protein. (Expected MW is 43.9 kDa)
Western Blot
Figure 3:Western blot analysis using BAK1 mAb against HEK293 (1) and BAK1 (AA: 29-187)-hIgGFc transfected HEK293 (2) cell lysate.
Flow cytometric
Figure 4:Flow cytometric analysis of Hela cells using BAK1 mouse mAb (green) and negative control (red).
Immunohistochemical analysis
Figure 5:Immunohistochemical analysis of paraffin-embedded bladder cancer tissues using BAK1 mouse mAb with DAB staining.
Immunohistochemical analysis
Figure 6:Immunohistochemical analysis of paraffin-embedded rectum cancer tissues using BAK1 mouse mAb with DAB staining.
For Research Use Only. Not for use in diagnostic procedures.