BAD Primary Antibody

Item Information
Catalog #Size/ConcentrationPrice
Specification
AliasesBBC2; BCL2L8
ProductOrderB
Clone#1G5B3
Entrez GenelD572
FormulationPurified antibody in PBS with 0.05% sodium azide.
HostMouse
IsotypeIgG1
ImmunogenPurified recombinant fragment of human BAD (AA: FULL(1-168)) expressed in E. Coli.
MW18.4kDa
Shipping InformationThis product will ship in a box containing blue ice at a temperature of 4°C. Learn More
Species ReactivityHuman
Application
ELISA1/10000
FCM (Flow Cytometry)1/200 - 1/400
WB (Western Blot)1/500 - 1/2000
Sequence
FULL(1-168)
Catalog#: 30637
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Images
Western Blot
Figure 1: Western blot analysis using BAD mAb against human BAD (AA: FULL(1-168)) recombinant protein. (Expected MW is 44.3 kDa)
Figure 1: Western blot analysis using BAD mAb against human BAD (AA: FULL(1-168)) recombinant protein. (Expected MW is 44.3 kDa)
Western Blot
Figure 2: Western blot analysis using BAD mAb against HEK293 (1) and BAD (AA: FULL(1-168))-hIgGFc transfected HEK293 (2) cell lysate.
Figure 2: Western blot analysis using BAD mAb against HEK293 (1) and BAD (AA: FULL(1-168))-hIgGFc transfected HEK293 (2) cell lysate.
Western Blot
Figure 3: Western blot analysis using BAD mouse mAb against MCF-7 (1), HEK293 (2) cell lysate.
Figure 3: Western blot analysis using BAD mouse mAb against MCF-7 (1), HEK293 (2) cell lysate.
Flow cytometric
Figure 4: Flow cytometric analysis of MCF-7 cells using BAD mouse mAb (green) and negative control (red).
Figure 4: Flow cytometric analysis of MCF-7 cells using BAD mouse mAb (green) and negative control (red).
Elisa
Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);
Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);
Product Overview
Description

The protein encoded by this gene is a member of the BCL-2 family. BCL-2 family members are known to be regulators of programmed cell death. This protein positively regulates cell apoptosis by forming heterodimers with BCL-xL and BCL-2, and reversing their death repressor activity. Proapoptotic activity of this protein is regulated through its phosphorylation. Protein kinases AKT and MAP kinase, as well as protein phosphatase calcineurin were found to be involved in the regulation of this protein. Alternative splicing of this gene results in two transcript variants which encode the same isoform.

References (references)
References (references)1. Clin Cancer Res. 2011 Oct 1;17(19):6356-66.
2. Mol Cancer Res. 2010 Aug;8(8):1116-25.