BACE1 Primary Antibody

Item Information
Catalog #Size/ConcentrationPrice
Specification
AliasesASP2; BACE; HSPC104
ProductOrderB
Clone#3C1C3
Entrez GenelD23621
FormulationPurified antibody in PBS with 0.05% sodium azide.
HostMouse
IsotypeIgG1
ImmunogenPurified recombinant fragment of human BACE1 (AA: 112-324) expressed in E. Coli.
MW55.7kDa
Shipping InformationThis product will ship in a box containing blue ice at a temperature of 4°C. Learn More
Species ReactivityHuman, Mouse, Rat
Application
ELISA1/10000
ICC (Immunocytochemistry)1/200 - 1/1000
FCM (Flow Cytometry)1/200 - 1/400
WB (Western Blot)1/500 - 1/2000
Sequence
112-324
Catalog#: 30625
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Images
Western Blot
Figure 1: Western blot analysis using BACE1 mAb against human BACE1 (AA: 112-324) recombinant protein. (Expected MW is 49.9 kDa)
Figure 1: Western blot analysis using BACE1 mAb against human BACE1 (AA: 112-324) recombinant protein. (Expected MW is 49.9 kDa)
Western Blot
Figure 2: Western blot analysis using BACE1 mAb against HEK293 (1) and BACE1 (AA: 112-324)-hIgGFc transfected HEK293 (2) cell lysate.
Figure 2: Western blot analysis using BACE1 mAb against HEK293 (1) and BACE1 (AA: 112-324)-hIgGFc transfected HEK293 (2) cell lysate.
Western Blot
Figure 3: Western blot analysis using BACE1 mouse mAb against Hela (1), SK-N-SH (2), HepG2 (3), C6 (4), PC-12 (5), PANC-1 (6), NIH/3T3 (7) cell lysate.
Figure 3: Western blot analysis using BACE1 mouse mAb against Hela (1), SK-N-SH (2), HepG2 (3), C6 (4), PC-12 (5), PANC-1 (6), NIH/3T3 (7) cell lysate.
Immunofluorescence analysis
Figure 4: Immunofluorescence analysis of MCF-7 cells using BACE1 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)
Figure 4: Immunofluorescence analysis of MCF-7 cells using BACE1 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)
Flow cytometric
Figure 5: Flow cytometric analysis of Hela cells using BACE1 mouse mAb (green) and negative control (red).
Figure 5: Flow cytometric analysis of Hela cells using BACE1 mouse mAb (green) and negative control (red).
Elisa
Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);
Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);
Product Overview
DescriptionCerebral deposition of amyloid beta peptide is an early and critical feature of Alzheimer's disease. Amyloid beta peptide is generated by proteolytic cleavage of amyloid precursor protein (APP) by two proteases, one of which is the protein encoded by this gene. The encoded protein, a member of the peptidase A1 protein family, is a type I integral membrane glycoprotein and aspartic protease that is found mainly in the Golgi. Multiple transcript variants encoding different isoforms have been described for this gene.

References (references)
References (references)1. J Neurochem. 2012 Jan;120 Suppl 1:62-70.
2. Eur J Neurosci. 2010 Oct;32(7):1223-38.