B2M Primary Antibody
|Formulation||Purified antibody in PBS with 0.05% sodium azide.|
|Immunogen||Purified recombinant fragment of human B2M (AA: 21-100) expressed in E. Coli.|
|Shipping Information||This product will ship in a box containing blue ice at a temperature of 4°C. Learn More|
|IHC_P(Immunohistochemistry)||1/200 - 1/1000|
|FCM (Flow Cytometry)||1/200 - 1/400|
|WB (Western Blot)||1/500 - 1/2000|
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Figure 1: Western blot analysis using B2M mAb against human B2M (AA: 21-100) recombinant protein. (Expected MW is 35.4 kDa)
Figure 2: Western blot analysis using B2M mAb against HEK293 (1) and B2M (AA: 21-100)-hIgGFc transfected HEK293 (2) cell lysate.
Figure 3: Western blot analysis using B2M mouse mAb against Hela (1), HEK293 (2), HepG2 (3),RAJI (4), A431 (5) and Jurkat (6) cell lysate.
Figure 4: Flow cytometric analysis of A431 cells using B2M mouse mAb (green) and negative control (red).
Figure 5: Immunohistochemical analysis of paraffin-embedded ovarian cancer tissues using B2M mouse mAb with DAB staining.
Figure 6: Immunohistochemical analysis of paraffin-embedded esophageal cancer tissues using B2M mouse mAb with DAB staining.
Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);
|Description||This gene encodes a serum protein found in association with the major histocompatibility complex (MHC) class I heavy chain on the surface of nearly all nucleated cells. The protein has a predominantly beta-pleated sheet structure that can form amyloid fibrils in some pathological conditions. A mutation in this gene has been shown to result in hypercatabolic hypoproteinemia. |
|References (references)||1. Cancer Immunol Immunother. 2012 Sep;61(9):1359-71. |
2. Lupus. 2012 Sep;21(10):1098-104.