AXIN1 Primary Antibody

Item Information

Catalog #

Size

Price

Description

This gene encodes a cytoplasmic protein which contains a regulation of G-protein signaling (RGS) domain and a dishevelled and axin (DIX) domain. The encoded protein interacts with adenomatosis polyposis coli, catenin beta-1, glycogen synthase kinase 3 beta, protein phosphate 2, and itself. This protein functions as a negative regulator of the wingless-type MMTV integration site family, member 1 (WNT) signaling pathway and can induce apoptosis. The crystal structure of a portion of this protein, alone and in a complex with other proteins, has been resolved. Mutations in this gene have been associated with hepatocellular carcinoma, hepatoblastomas, ovarian endometriod adenocarcinomas, and medullablastomas. Alternative splicing results in multiple transcript variants.

Product Overview

Entrez GenelD

8312

Aliases

AXIN; PPP1R49

Clone#

4E9F1

Host / Isotype

Mouse / IgG2b

Species Reactivity

Human

Immunogen

Purified recombinant fragment of human AXIN1 (AA: 546-752) expressed in E. Coli.

Formulation

Purified antibody in PBS with 0.05% sodium azide

Storage

4°C; -20°C for long term storage

Product Applications

WB (Western Blot)

1/500 - 1/2000

ICC (Immunocytochemistry)

1/50 - 1/250

FCM (Flow Cytometry)

1/200 - 1/400

ELISA

1/10000

References

1.Cancer Lett. 2014 Dec 1;355(1):1-8.
2.BMC Cancer. 2013 Aug 2;13:368.

Product Image

Elisa

Figure 1: Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)

Western Blot

Figure 2:Western blot analysis using AXIN1 mAb against human AXIN1 (AA: 546-752) recombinant protein. (Expected MW is 48.7 kDa)

Western Blot

Figure 3:Western blot analysis using AXIN1 mAb against HEK293 (1) and AXIN1 (AA: 546-752)-hIgGFc transfected HEK293 (2) cell lysate.

Immunofluorescence analysis

Figure 4:Immunofluorescence analysis of Hela cells using AXIN1 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)

Flow cytometric