AURKA Primary Antibody
The protein encoded by this gene is a cell cycle-regulated kinase that appears to be involved in microtubule formation and/or stabilization at the spindle pole during chromosome segregation. The encoded protein is found at the centrosome in interphase cells and at the spindle poles in mitosis. This gene may play a role in tumor development and progression. A processed pseudogene of this gene has been found on chromosome 1, and an unprocessed pseudogene has been found on chromosome 10. Multiple transcript variants encoding the same protein have been found for this gene.
Purified recombinant fragment of human AURKA (AA: 268-404) expressed in E. Coli.
Purified antibody in PBS with 0.05% sodium azide
4°C; -20°C for long term storage
Figure 1: Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
Figure 2:Western blot analysis using AURKA mAb against human AURKA (AA: 268-404) recombinant protein. (Expected MW is 41.5 kDa)
Figure 3:Western blot analysis using AURKA mAb against HEK293 (1) and AURKA (AA: 268-404)-hIgGFc transfected HEK293 (2) cell lysate.
Figure 4:Western blot analysis using AURKA mouse mAb against HEK293 (1), PC-12 (2), and HT-29 (3) cell lysate.
For Research Use Only. Not for use in diagnostic procedures.