ATP6V0A4
This gene encodes a component of vacuolar ATPase (V-ATPase), a multisubunit enzyme that mediates acidification of intracellular compartments of eukaryotic cells. V-ATPase dependent acidification is necessary for such intracellular processes as protein sorting, zymogen activation, receptor-mediated endocytosis, and synaptic vesicle proton gradient generation. V-ATPase is composed of a cytosolic V1 domain and a transmembrane V0 domain. The V1 domain consists of three A and three B subunits, two G subunits plus the C, D, E, F, and H subunits. The V1 domain contains the ATP catalytic site. The V0 domain consists of five different subunits: a, c, c', c'', and d. This gene is one of four genes in man and mouse that encode different isoforms of the a subunit. Alternatively spliced transcript variants encoding the same protein have been described. Mutations in this gene are associated with renal tubular acidosis associated with preserved hearing.
2.J Genet. 2014 Dec;93(3):859-63.
![Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)](https://cdn.shopify.com/s/files/1/0604/2661/7019/products/32340ea.jpg?v=1669029592)
![Figure 2:Western blot analysis using ATP6V0A4 mAb against human ATP6V0A4 (AA: 228-390) recombinant protein. (Expected MW is 44.5 kDa)](https://cdn.shopify.com/s/files/1/0604/2661/7019/products/32340rwb.jpg?v=1669029592)
![Figure 3:Western blot analysis using ATP6V0A4 mAb against HEK293-6e (1) and ATP6V0A4 (AA: 228-390)-hIgGFc transfected HEK293-6e (2) cell lysate.](https://cdn.shopify.com/s/files/1/0604/2661/7019/products/32340twb.jpg?v=1669029592)
![Figure 4:Flow cytometric analysis of Hela cells using ATP6V0A4 mouse mAb (green) and negative control (red).](https://cdn.shopify.com/s/files/1/0604/2661/7019/products/32340fcm.jpg?v=1669029592)
![Figure 5:Immunohistochemical analysis of paraffin-embedded breast cancer tissues using ATP6V0A4 mouse mAb with DAB staining.](https://cdn.shopify.com/s/files/1/0604/2661/7019/products/32340ihc.jpg?v=1669029592)
![Figure 6:Immunohistochemical analysis of paraffin-embedded human kidney tissues using ATP6V0A4 mouse mAb with DAB staining.](https://cdn.shopify.com/s/files/1/0604/2661/7019/products/32340-1ihc.jpg?v=1669029592)