Mouse Monoclonal Antibody to ATP

Item Information
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Description

This gene encodes a subunit of mitochondrial ATP synthase. Mitochondrial ATP synthase catalyzes ATP synthesis, using an electrochemical gradient of protons across the inner membrane during oxidative phosphorylation. ATP synthase is composed of two linked multi-subunit complexes: the soluble catalytic core, F1, and the membrane-spanning component, Fo, comprising the proton channel. The catalytic portion of mitochondrial ATP synthase consists of 5 different subunits (alpha, beta, gamma, delta, and epsilon) assembled with a stoichiometry of 3 alpha, 3 beta, and a single representative of the other 3. The proton channel consists of three main subunits (a, b, c). This gene encodes the alpha subunit of the catalytic core. Alternatively spliced transcript variants encoding the different isoforms have been identified. Pseudogenes of this gene are located on chromosomes 9, 2, and 16. [provided by RefSeq, Mar 2012]

Product Overview
Entrez GenelD
498
Aliases
OMR; ORM; ATPM; MOM2; ATP5A; hATP1; ATP5A1; MC5DN4; ATP5AL2; COXPD22; HEL-S-123m
Clone#
2F2C1
Host / Isotype
Mouse / IgG1
Immunogen
Purified recombinant fragment of human ATP (AA: 44-220) expressed in E. Coli.
Formulation
Purified antibody in PBS with 0.05% sodium azide
Storage
4℃; -20℃ for long term storage
Product Applications
WB (Western Blot)
Western Blot.
FCM (Flow Cytometry)
1/200 - 1/400
ELISA
1/10000
References
1,Oncol Rep. 2018 Feb;39(2):525-536.2,Oxid Med Cell Longev. 2018 Nov 14;2018:1347174.
Product Image
Elisa
Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
Flow cytometric analysis
Figure 2:Flow cytometric analysis of Hela cells using ATP mouse mAb (green) and negative control (red).
Figure 2:Flow cytometric analysis of Hela cells using ATP mouse mAb (green) and negative control (red).
For Research Use Only. Not for use in diagnostic procedures.