ATG7 Primary Antibody
Item Information
Catalog #
Size
Price
Description
This gene encodes an E1-like activating enzyme that is essential for autophagy and cytoplasmic to vacuole transport. The encoded protein is also thought to modulate p53-dependent cell cycle pathways during prolonged metabolic stress. It has been associated with multiple functions, including axon membrane trafficking, axonal homeostasis, mitophagy, adipose differentiation, and hematopoietic stem cell maintenance. Alternative splicing results in multiple transcript variants.
Product Overview
Entrez GenelD
10533
Aliases
GSA7; APG7L; APG7-LIKE
Clone#
7D3E5
Host / Isotype
Mouse / IgG2b
Species Reactivity
Human
Immunogen
Purified recombinant fragment of human ATG7 (AA: 558-703) expressed in E. Coli.
Formulation
Purified antibody in PBS with 0.05% sodium azide
Storage
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Product Applications
WB (Western Blot)
1/500 - 1/2000
FCM (Flow Cytometry)
1/200 - 1/400
ELISA
1/10000
References
1.Oncotarget. 2014 Mar 30;5(6):1526-37.
2.J Biol Chem. 2012 Oct 12;287(42):35576-88.
2.J Biol Chem. 2012 Oct 12;287(42):35576-88.
Product Image
Elisa
Figure 1: Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
Western Blot
Figure 2:Western blot analysis using ATG7 mAb against human ATG7 (AA: 558-703) recombinant protein. (Expected MW is 41.9 kDa)
Western Blot
Figure 3:Western blot analysis using ATG7 mAb against HEK293 (1) and ATG7 (AA: 558-703)-hIgGFc transfected HEK293 (2) cell lysate.
Flow cytometric
Figure 4:Flow cytometric analysis of HeLa cells using ATG7 mouse mAb (green) and negative control (red).
For Research Use Only. Not for use in diagnostic procedures.