ATG7 Primary Antibody
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Description
This gene encodes an E1-like activating enzyme that is essential for autophagy and cytoplasmic to vacuole transport. The encoded protein is also thought to modulate p53-dependent cell cycle pathways during prolonged metabolic stress. It has been associated with multiple functions, including axon membrane trafficking, axonal homeostasis, mitophagy, adipose differentiation, and hematopoietic stem cell maintenance. Alternative splicing results in multiple transcript variants.
Product Overview
Immunogen
Purified recombinant fragment of human ATG7 (AA: 558-703) expressed in E. Coli.
Formulation
Purified antibody in PBS with 0.05% sodium azide
Storage
4°C; -20°C for long term storage
Product Image
Elisa
Figure 1: Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
Western Blot
Figure 2:Western blot analysis using ATG7 mAb against human ATG7 (AA: 558-703) recombinant protein. (Expected MW is 41.9 kDa)
Western Blot
Figure 3:Western blot analysis using ATG7 mAb against HEK293 (1) and ATG7 (AA: 558-703)-hIgGFc transfected HEK293 (2) cell lysate.
Flow cytometric
Figure 4:Flow cytometric analysis of HeLa cells using ATG7 mouse mAb (green) and negative control (red).
For Research Use Only. Not for use in diagnostic procedures.