ATG4A Primary Antibody
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Autophagy is the process by which endogenous proteins and damaged organelles are destroyed intracellularly. Autophagy is postulated to be essential for cell homeostasis and cell remodeling during differentiation, metamorphosis, non-apoptotic cell death, and aging. Reduced levels of autophagy have been described in some malignant tumors, and a role for autophagy in controlling the unregulated cell growth linked to cancer has been proposed. This gene encodes a member of the autophagin protein family. The encoded protein is also designated as a member of the C-54 family of cysteine proteases.
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Formulation
Purified antibody in PBS with 0.05% sodium azide
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Elisa

Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
Western Blot

Figure 2:Western blot analysis using ATG4A mAb against human ATG4A (AA: 258-398) recombinant protein. (Expected MW is 42.2 kDa)
Western Blot

Figure 3:Western blot analysis using ATG4A mAb against HEK293 (1) and ATG4A (AA: 258-398)-hIgGFc transfected HEK293 (2) cell lysate.
Flow cytometric

Figure 4:Flow cytometric analysis of K562 cells using ATG4A mouse mAb (green) and negative control (red).
For Research Use Only. Not for use in diagnostic procedures.