ARFGAP1 Primary Antibody
Item Information
Catalog #
Size
Price
Description
The protein encoded by this gene is a GTPase-activating protein, which associates with the Golgi apparatus and which interacts with ADP-ribosylation factor 1. The encoded protein promotes hydrolysis of ADP-ribosylation factor 1-bound GTP and is required for the dissociation of coat proteins from Golgi-derived membranes and vesicles. Dissociation of the coat proteins is required for the fusion of these vesicles with target compartments. The activity of this protein is stimulated by phosphoinosides and inhibited by phosphatidylcholine. Alternative splicing results in multiple transcript variants.
Product Overview
Entrez GenelD
55738
Aliases
ARF1GAP; HRIHFB2281
Clone#
1C4E2
Host / Isotype
Mouse / IgG2b
Species Reactivity
Human, Mouse
Immunogen
Purified recombinant fragment of human ARFGAP1 (AA: 270-414) expressed in E. Coli.
Formulation
Purified antibody in PBS with 0.05% sodium azide
Storage
4°C; -20°C for long term storage
Product Applications
WB (Western Blot)
1/500 - 1/2000
FCM (Flow Cytometry)
1/200 - 1/400
ELISA
1/10000
References
1.PLoS One. 2014 Nov 14;9(11):e111309.
2.Methods Enzymol. 2001;329:307-16.
2.Methods Enzymol. 2001;329:307-16.
Product Image
Elisa
Figure 1: Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
Western Blot
Figure 2:Western blot analysis using ARFGAP1 mAb against human ARFGAP1 (AA: 270-414) recombinant protein. (Expected MW is 41.5 kDa)
Western Blot
Figure 3:Western blot analysis using ARFGAP1 mAb against HEK293 (1) and ARFGAP1 (AA: 270-414)-hIgGFc transfected HEK293 (2) cell lysate.
Western Blot
Figure 4:Western blot analysis using ARFGAP1 mouse mAb against MOLT4 (1), C2C12 (2), HepG2 (3), MCF-7 (4), and Lncap (5) cell lysate.
Flow cytometric
Figure 5:Flow cytometric analysis of HepG2 cells using ARFGAP1 mouse mAb (green) and negative control (red).
For Research Use Only. Not for use in diagnostic procedures.