APP Primary Antibody

Item Information
Catalog #Size/ConcentrationPrice
Specification
AliasesAAA; AD1; PN2; ABPP; APPI; CVAP; ABETA; PN-II; CTFgamma
ProductOrderA
Clone#5H10A10
Entrez GenelD351
FormulationPurified antibody in PBS with 0.05% sodium azide.
HostMouse
IsotypeIgG2b
ImmunogenPurified recombinant fragment of human APP (AA: 483-699) expressed in E. Coli.
MW87kDa
Shipping InformationThis product will ship in a box containing blue ice at a temperature of 4°C. Learn More
Species ReactivityHuman
Application
ELISA1/10000
FCM (Flow Cytometry)1/200 - 1/400
WB (Western Blot)1/500 - 1/2000
Sequence
483-699
Catalog#: 30709
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Images
Western Blot
Figure 1: Western blot analysis using APP mAb against human APP (AA: 483-699) recombinant protein. (Expected MW is 50.7 kDa)
Figure 1: Western blot analysis using APP mAb against human APP (AA: 483-699) recombinant protein. (Expected MW is 50.7 kDa)
Western Blot
Figure 2: Western blot analysis using APP mAb against HEK293 (1) and APP (AA: 483-699)-hIgGFc transfected HEK293 (2) cell lysate.
Figure 2: Western blot analysis using APP mAb against HEK293 (1) and APP (AA: 483-699)-hIgGFc transfected HEK293 (2) cell lysate.
Flow cytometric
Figure 3: Flow cytometric analysis of Hela cells using APP mouse mAb (green) and negative control (red).
Figure 3: Flow cytometric analysis of Hela cells using APP mouse mAb (green) and negative control (red).
Elisa
Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);
Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);
Product Overview
Description

This gene encodes a cell surface receptor and transmembrane precursor protein that is cleaved by secretases to form a number of peptides. Some of these peptides are secreted and can bind to the acetyltransferase complex APBB1/TIP60 to promote transcriptional activation, while others form the protein basis of the amyloid plaques found in the brains of patients with Alzheimer disease. Mutations in this gene have been implicated in autosomal dominant Alzheimer disease and cerebroarterial amyloidosis (cerebral amyloid angiopathy). Multiple transcript variants encoding several different isoforms have been found for this gene.

References (references)
References (references)1. Proc Natl Acad Sci U S A. 2013 Sep 3;110(36):14604-9.
2. ACS Chem Neurosci. 2013 Mar 20;4(3):454-62.