APP Primary Antibody

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Description

This gene encodes a cell surface receptor and transmembrane precursor protein that is cleaved by secretases to form a number of peptides. Some of these peptides are secreted and can bind to the acetyltransferase complex APBB1/TIP60 to promote transcriptional activation, while others form the protein basis of the amyloid plaques found in the brains of patients with Alzheimer disease. Mutations in this gene have been implicated in autosomal dominant Alzheimer disease and cerebroarterial amyloidosis (cerebral amyloid angiopathy). Multiple transcript variants encoding several different isoforms have been found for this gene.

Product Overview
Formulation
Purified antibody in PBS with 0.05% sodium azide.
Storage
4°C; -20°C for long term storage
Product Image
Western Blot
Figure 1: Western blot analysis using APP mAb against human APP (AA: 483-699) recombinant protein. (Expected MW is 50.7 kDa)
Figure 1: Western blot analysis using APP mAb against human APP (AA: 483-699) recombinant protein. (Expected MW is 50.7 kDa)
Western Blot
Figure 2: Western blot analysis using APP mAb against HEK293 (1) and APP (AA: 483-699)-hIgGFc transfected HEK293 (2) cell lysate.
Figure 2: Western blot analysis using APP mAb against HEK293 (1) and APP (AA: 483-699)-hIgGFc transfected HEK293 (2) cell lysate.
Immunofluorescence analysis
Figure 3: Immunofluorescence analysis of A431 cells using APP mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)
Figure 3: Immunofluorescence analysis of A431 cells using APP mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)
Elisa
Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);
Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);
For Research Use Only. Not for use in diagnostic procedures.