ApoB Primary Antibody
This gene product is the main apolipoprotein of chylomicrons and low density lipoproteins. It occurs in plasma as two main isoforms, apoB-48 and apoB-100: the former is synthesized exclusively in the gut and the latter in the liver. The intestinal and the hepatic forms of apoB are encoded by a single gene from a single, very long mRNA. The two isoforms share a common N-terminal sequence. The shorter apoB-48 protein is produced after RNA editing of the apoB-100 transcript at residue 2180 (CAA->UAA), resulting in the creation of a stop codon, and early translation termination. Mutations in this gene or its regulatory region cause hypobetalipoproteinemia, normotriglyceridemic hypobetalipoproteinemia, and hypercholesterolemia due to ligand-defective apoB, diseases affecting plasma cholesterol and apoB levels.
2. Atherosclerosis. 2009 Sep;206(1):17-30.
![Figure 1: Western blot analysis using ApoB mAb against human ApoB (AA: 3900-4110) recombinant protein. (Expected MW is 45 kDa)](https://cdn.shopify.com/s/files/1/0604/2661/7019/products/30214rwb.jpg?v=1666699389)
![Figure 2: Immunofluorescence analysis of HepG2 cells using ApoB mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.](https://cdn.shopify.com/s/files/1/0604/2661/7019/products/30214icc.jpg?v=1666699389)
![Figure 3: Flow cytometric analysis of serun using ApoB mouse mAb (green) and negative control (purple).](https://cdn.shopify.com/s/files/1/0604/2661/7019/products/30214fcm.jpg?v=1666699389)
![Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);](https://cdn.shopify.com/s/files/1/0604/2661/7019/products/30214ea.jpg?v=1666699389)