APEX1 Primary Antibody
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Description
Apurinic/apyrimidinic (AP) sites occur frequently in DNA molecules by spontaneous hydrolysis, by DNA damaging agents or by DNA glycosylases that remove specific abnormal bases. AP sites are pre-mutagenic lesions that can prevent normal DNA replication so the cell contains systems to identify and repair such sites. Class II AP endonucleases cleave the phosphodiester backbone 5' to the AP site. This gene encodes the major AP endonuclease in human cells. Splice variants have been found for this gene; all encode the same protein.
Product Overview
Formulation
Purified antibody in PBS with 0.05% sodium azide
Storage
4°C; -20°C for long term storage
Product Image
Elisa
Figure 1: Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
Western Blot
Figure 2:Western blot analysis using APEX1 mAb against human APEX1 (AA: 219-318) recombinant protein. (Expected MW is 37.4 kDa)
Western Blot
Figure 3:Western blot analysis using APEX1 mAb against HEK293 (1) and APEX1 (AA: 219-318)-hIgGFc transfected HEK293 (2) cell lysate.
Western Blot
Figure 4:Western blot analysis using APEX1 mouse mAb against Hela (1), Jurkat (2), SW480 (3), A431 (4), HepG2 (5), NIH/3T3 (6), and PC-12 (7) cell lysate.
Flow cytometric
Figure 5:Flow cytometric analysis of HeLa cells using APEX1 mouse mAb (green) and negative control (red).
Flow cytometric
Figure 6:Flow cytometric analysis of SK-N-SH cells using APEX1 mouse mAb (green) and negative control (red).
Immunohistochemical analysis
Figure 7:Immunohistochemical analysis of paraffin-embedded breast cancer tissues using APEX1 mouse mAb with DAB staining.
Immunohistochemical analysis
Figure 8:Immunohistochemical analysis of paraffin-embedded esophageal cancer tissues using APEX1 mouse mAb with DAB staining.
For Research Use Only. Not for use in diagnostic procedures.