APAF1 Primary Antibody
Item Information
Catalog #
Size
Price
Description
This gene encodes a cytoplasmic protein that initiates apoptosis. This protein contains several copies of the WD-40 domain, a caspase recruitment domain (CARD), and an ATPase domain (NB-ARC). Upon binding cytochrome c and dATP, this protein forms an oligomeric apoptosome. The apoptosome binds and cleaves caspase 9 preproprotein, releasing its mature, activated form. Activated caspase 9 stimulates the subsequent caspase cascade that commits the cell to apoptosis. Alternative splicing results in several transcript variants encoding different isoforms.
Product Overview
Entrez GenelD
317
Aliases
CED4; APAF-1
Clone#
2H9A1
Host / Isotype
Mouse / IgG2b
Species Reactivity
Human
Immunogen
Purified recombinant fragment of human APAF1 (AA: 1138-1237) expressed in E. Coli.
Formulation
Purified antibody in PBS with 0.05% sodium azide
Storage
4°C; -20°C for long term storage
Product Applications
WB (Western Blot)
1/500 - 1/2000
ICC (Immunocytochemistry)
1/100 - 1/500
FCM (Flow Cytometry)
1/200 - 1/400
ELISA
1/10000
References
1.Tumour Biol. 2014 Mar;35(3):2211-8.
2.Cancer Sci. 2011 Jan;102(1):267-74.
2.Cancer Sci. 2011 Jan;102(1):267-74.
Product Image
Elisa
Figure 1: Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
Western Blot
Figure 2:Western blot analysis using APAF1 mAb against human APAF1 (AA: 1138-1237) recombinant protein. (Expected MW is 37 kDa)
Western Blot
Figure 3:Western blot analysis using APAF1 mAb against HEK293 (1) and APAF1 (AA: 1138-1237)-hIgGFc transfected HEK293 (2) cell lysate.
Immunofluorescence analysis
Figure 4:Immunofluorescence analysis of Hela cells using APAF1 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)
Flow cytometric
Figure 5:Flow cytometric analysis of Hela cells using APAF1 mouse mAb (green) and negative control (red).
For Research Use Only. Not for use in diagnostic procedures.