ALPL Primary Antibody

Item Information
Catalog #Size/ConcentrationPrice
Specification
AliasesHOPS; TNAP; APTNAP; TNSALP; AP-TNAP
ProductOrderA
Clone#2F4
Entrez GenelD249
FormulationPurified antibody in PBS with 0.05% sodium azide
HostMouse
IsotypeIgG1
ImmunogenPurified recombinant fragment of human ALPL expressed in E. Coli.
MW57.3kDa
Shipping InformationThis product will ship in a box containing blue ice at a temperature of 4°C. Learn More
Species ReactivityHuman
Application
ELISA1/10000
IHC_P(Immunohistochemistry)1/200 - 1/1000
FCM (Flow Cytometry)1/200 - 1/400
WB (Western Blot)1/500 - 1/2000
Sequence
18-502
Catalog#: 30337
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Images
Western Blot
Figure 1: Western blot analysis using ALPL mAb against human ALPL (AA: 18-502) recombinant protein. (Expected MW is 78.9 kDa)
Figure 1: Western blot analysis using ALPL mAb against human ALPL (AA: 18-502) recombinant protein. (Expected MW is 78.9 kDa)
Western Blot
Figure 2: Western blot analysis using ALPL mouse mAb against HeLa (1), and NTERA-2 (4) cell lysate.
Figure 2: Western blot analysis using ALPL mouse mAb against HeLa (1), and NTERA-2 (4) cell lysate.
Immunohistochemical analysis
Figure 3: Immunohistochemical analysis of paraffin-embedded ovarian cancer tissues using ALPL mouse mAb with DAB staining.
Figure 3: Immunohistochemical analysis of paraffin-embedded ovarian cancer tissues using ALPL mouse mAb with DAB staining.
Flow cytometric
Figure 4: Flow cytometric analysis of MCF-7 cells using ALPL mouse mAb (green) and negative control (red).
Figure 4: Flow cytometric analysis of MCF-7 cells using ALPL mouse mAb (green) and negative control (red).
Elisa
Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);
Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);
Product Overview
Description

There are at least four distinct but related alkaline phosphatases: intestinal, placental, placental-like, and liver/bone/kidney (tissue non-specific). The first three are located together on chromosome 2, while the tissue non-specific form is located on chromosome 1. The product of this gene is a membrane bound glycosylated enzyme that is not expressed in any particular tissue and is, therefore, referred to as the tissue-nonspecific form of the enzyme. The exact physiological function of the alkaline phosphatases is not known. A proposed function of this form of the enzyme is matrix mineralization; however, mice that lack a functional form of this enzyme show normal skeletal development. This enzyme has been linked directly to hypophosphatasia, a disorder that is characterized by hypercalcemia and includes skeletal defects. The character of this disorder can vary, however, depending on the specific mutation since this determines age of onset and severity of symptoms. Alternatively spliced transcript variants have been described.

References (references)
References (references)J Rheumatol. 2009 Dec;36(12):2758-65.
Calcif Tissue Int. 2009 Sep;85(3):228-34.