AKT2 Primary Antibody

Item Information
Catalog #Size/ConcentrationPrice
Specification
AliasesPKBB; PRKBB; PKBBETA; RAC-BETA
ProductOrderA
Clone#1B6
Entrez GenelD208
FormulationAscitic fluid containing 0.03% sodium azide.
HostMouse
IsotypeIgG2b
ImmunogenPurified recombinant fragment of human AKT2 expressed in E. Coli.
MW60kDa
Shipping InformationThis product will ship in a box containing blue ice at a temperature of 4°C. Learn More
Species ReactivityHuman, Rat, Monkey
Application
ELISA1/10000
ICC (Immunocytochemistry)1/200 - 1/1000
IHC_P(Immunohistochemistry)1/200 - 1/1000
WB (Western Blot)1/500 - 1/2000
Sequence
2-149
Catalog#: 30002
Inquire
Contact us to order
Images
Western Blot
Figure 1: Western blot analysis using AKT2 mouse mAb against A431 (1), Jurkat (2), HEK293 (3), A549 (4), MCF-7 (5) and PC-12 (6) cell lysate.
Figure 1: Western blot analysis using AKT2 mouse mAb against A431 (1), Jurkat (2), HEK293 (3), A549 (4), MCF-7 (5) and PC-12 (6) cell lysate.
Immunohistochemical analysis
Figure 2: Immunohistochemical analysis of paraffin-embedded human Thymus tissues using anti-AKT2 mAb
Figure 2: Immunohistochemical analysis of paraffin-embedded human Thymus tissues using anti-AKT2 mAb
Immunofluorescence analysis
Figure 3: Immunofluorescence analysis of PANC-1 (left) and Hela (right) cells using AKT2 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
Figure 3: Immunofluorescence analysis of PANC-1 (left) and Hela (right) cells using AKT2 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
Product Overview
Description

Akt2 (also designated protein kinase B beta or v-akt murine thymoma viral oncogene homolog 2 ), with 481-amino acid protein (about 53kDa), belongs to the AKT serine/threonine protein kinase family, which also includes Akt1 and Akt3. They are involved in a wide variety of biological processes including cell proliferation, differentiation, apoptosis, tumorigenesis, as well as glycogen synthesis and glucose uptake. Among the members of AKT family, Akt2 is associated with the development of human cancers. Akt2 inhibits cisplatin-induced JNK/p38 and Bax activation through phosphorylation of ASK1 and thus, plays an important role in chemoresistance. Further, Akt2 plays a specific role in muscle differentiation.

References (references)
References (references)1. Am J Physiol Endocrinol Metab. 2004 Jul;287(1):E8-E15.
2. Oncol Rep. 2004 Jan;11(1):25-32.
3. Cancer Res. 2003 Jan 1;63(1):196-206.