AKT1 Primary Antibody
Item Information
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Description
The serine-threonine protein kinase encoded by the AKT1 gene is catalytically inactive in serum-starved primary and immortalized fibroblasts. AKT1 and the related AKT2 are activated by platelet-derived growth factor. The activation is rapid and specific, and it is abrogated by mutations in the pleckstrin homology domain of AKT1. It was shown that the activation occurs through phosphatidylinositol 3-kinase. In the developing nervous system AKT is a critical mediator of growth factor-induced neuronal survival. Survival factors can suppress apoptosis in a transcription-independent manner by activating the serine/threonine kinase AKT1, which then phosphorylates and inactivates components of the apoptotic machinery. Mutations in this gene have been associated with the Proteus syndrome. Multiple alternatively spliced transcript variants have been found for this gene.
Product Overview
Entrez GenelD
207
Aliases
AKT; PKB; RAC; CWS6; PRKBA; PKB-ALPHA; RAC-ALPHA
Clone#
1F7E10
Host / Isotype
Mouse / IgG1
Species Reactivity
Human, Mouse, Monkey, Rat
Immunogen
Purified recombinant fragment of human AKT1 (AA: 1-150) expressed in E. Coli.
Formulation
Purified antibody in PBS with 0.05% sodium azide
Storage
4°C; -20°C for long term storage
Product Applications
WB (Western Blot)
1/500 - 1/2000
ICC (Immunocytochemistry)
1/200 - 1/1000
FCM (Flow Cytometry)
1/200 - 1/400
ELISA
1/10000
References
1.Sci Rep. 2015 Jan 13;5:7758.
2.J Cancer Res Clin Oncol. 2015 Apr;141(4):615-26.
2.J Cancer Res Clin Oncol. 2015 Apr;141(4):615-26.
Product Image
Elisa
Figure 1: Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
Western Blot
Figure 2:Western blot analysis using AKT1 mAb against human AKT1 (AA: 1-150) recombinant protein. (Expected MW is 43.6 kDa)
Western Blot
Figure 3:Western blot analysis using AKT1 mAb against HEK293 (1) and AKT1 (AA: 1-150)-hIgGFc transfected HEK293 (2) cell lysate.
Western Blot
Figure 4:Western blot analysis using AKT1 mouse mAb against MCF-7 (1), NIH/3T3 (2), Hela (3), COS7 (4), and C6 (5) cell lysate.
Immunofluorescence analysis
Figure 5:Immunofluorescence analysis of HepG2 cells using AKT1 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)
Flow cytometric
Figure 6:Flow cytometric analysis of Hela cells using AKT1 mouse mAb (green) and negative control (red).
For Research Use Only. Not for use in diagnostic procedures.