AIM2 Primary Antibody
Item Information
Catalog #
Size
Price
Description
AIM2 is a member of the IFI20X /IFI16 family. It plays a putative role in tumorigenic reversion and may control cell proliferation. Interferon-gamma induces expression of AIM2.
Product Overview
Entrez GenelD
9447
Aliases
PYHIN4
Clone#
3C4G11
Host / Isotype
Mouse / IgG1
Species Reactivity
Human
Immunogen
Purified recombinant fragment of human AIM2 (AA: 1-195) expressed in E. Coli.
Formulation
Purified antibody from tissue culture in PBS with 0.05% sodium azide
Storage
4°C; -20°C for long term storage
Product Applications
WB (Western Blot)
1/500 - 1/2000
ICC (Immunocytochemistry)
1/200 - 1/1000
FCM (Flow Cytometry)
1/200 - 1/400
ELISA
1/10000
References
1.Mol Cancer Res. 2013 Oct;11(10):1193-202.
2.Int J Cancer. 2010 Apr 15;126(8):1838-49.
2.Int J Cancer. 2010 Apr 15;126(8):1838-49.
Product Image
Western Blot
Figure 1:Western blot analysis using AIM2 mAb against human AIM2 (AA: 1-195) recombinant protein. (Expected MW is 48.2 kDa)
Elisa
Figure 2:Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);
Western Blot
Figure 3:Western blot analysis using AIM2 mAb against HEK293 (1) and AIM2 (AA: 1-195)-hIgGFc transfected HEK293 (2) cell lysate.
Immunofluorescence analysis
Figure 4:Immunofluorescence analysis of A549 cells using AIM2 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)
Immunofluorescence analysis
Figure 5:Immunofluorescence analysis of MCF-7 cells using AIM2 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)
Flow cytometric
Figure 6:Flow cytometric analysis of Hela cells using AIM2 mouse mAb (green) and negative control (red).
For Research Use Only. Not for use in diagnostic procedures.