AIF Primary Antibody

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Description

This gene encodes a flavoprotein essential for nuclear disassembly in apoptotic cells, and it is found in the mitochondrial intermembrane space in healthy cells. Induction of apoptosis results in the translocation of this protein to the nucleus where it affects chromosome condensation and fragmentation. In addition, this gene product induces mitochondria to release the apoptogenic proteins cytochrome c and caspase-9. Mutations in this gene cause combined oxidative phosphorylation deficiency 6, which results in a severe mitochondrial encephalomyopathy. Alternative splicing results in multiple transcript variants. A related pseudogene has been identified on chromosome 10

Product Overview
Entrez GenelD
9131
Aliases
AIFM1; AIF; PDCD8; COXPD6; MGC111425
Clone#
4E7
Host / Isotype
Mouse / IgG2b
Species Reactivity
Human, Mouse, Monkey, Rat
Immunogen
Purified recombinant fragment of human AIF expressed in E. Coli.
Formulation
Ascitic fluid containing 0.03% sodium azide.
Storage
4°C; -20°C for long term storage
Product Applications
WB (Western Blot)
1/500 - 1/2000
IHC_P(Immunohistochemistry)
1/200 - 1/1000
ICC (Immunocytochemistry)
1/200 - 1/1000
FCM (Flow Cytometry)
1/200 - 1/400
ELISA
1/10000
References
1. Apoptosis. 2009 Jun;14(6):796-808.
2. PLoS One. 2009;4(2):e4394. Epub 2009 Feb 6.
Product Image
Western Blot
Figure 1: Western blot analysis using AIF mAb against human AIF (AA: 1-261) recombinant protein. (Expected MW is 35.6 kDa)
Figure 1: Western blot analysis using AIF mAb against human AIF (AA: 1-261) recombinant protein. (Expected MW is 35.6 kDa)
Western Blot
Figure 2: Western blot analysis using AIF mouse mAb against NIH/3T3 (1), Jurkat (2), Hela (3), HepG2 (4), MOLT4 (5), C6 (6), RAJI (7), Cos7 (8) and PC-12 (9) cell lysate.
Figure 2: Western blot analysis using AIF mouse mAb against NIH/3T3 (1), Jurkat (2), Hela (3), HepG2 (4), MOLT4 (5), C6 (6), RAJI (7), Cos7 (8) and PC-12 (9) cell lysate.
Immunohistochemical analysis
Figure 3: Immunohistochemical analysis of paraffin-embedded human breast cancer tissues using AIF mouse mAb with DAB staining.
Figure 3: Immunohistochemical analysis of paraffin-embedded human breast cancer tissues using AIF mouse mAb with DAB staining.
Immunohistochemical analysis
Figure 4: Immunohistochemical analysis of paraffin-embedded human cervical cancer tissues using AIF mouse mAb with DAB staining.
Figure 4: Immunohistochemical analysis of paraffin-embedded human cervical cancer tissues using AIF mouse mAb with DAB staining.
Immunohistochemical analysis
Figure 5: Immunohistochemical analysis of paraffin-embedded human rectum cancer tissues using AIF mouse mAb with DAB staining.
Figure 5: Immunohistochemical analysis of paraffin-embedded human rectum cancer tissues using AIF mouse mAb with DAB staining.
Immunohistochemical analysis
Figure 6: Immunohistochemical analysis of paraffin-embedded human lung cancer tissues using AIF mouse mAb with DAB staining.
Figure 6: Immunohistochemical analysis of paraffin-embedded human lung cancer tissues using AIF mouse mAb with DAB staining.
Immunofluorescence analysis
Figure 7: Immunofluorescence analysis of NIH/3T3 cells using AIF mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
Figure 7: Immunofluorescence analysis of NIH/3T3 cells using AIF mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
Flow cytometric
Figure 8: Flow cytometric analysis of HepG2 cells using AIF mouse mAb (blue) and negative control (red).
Figure 8: Flow cytometric analysis of HepG2 cells using AIF mouse mAb (blue) and negative control (red).
Elisa
Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);
Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);
For Research Use Only. Not for use in diagnostic procedures.