AIF Primary Antibody
|Aliases||AIFM1; AIF; PDCD8; COXPD6; MGC111425|
|Formulation||Ascitic fluid containing 0.03% sodium azide. |
|Immunogen||Purified recombinant fragment of human AIF expressed in E. Coli. |
|Shipping Information||This product will ship in a box containing blue ice at a temperature of 4°C. Learn More|
|Species Reactivity||Human, Mouse, Monkey, Rat|
|ICC (Immunocytochemistry)||1/200 - 1/1000|
|IHC_P(Immunohistochemistry)||1/200 - 1/1000|
|FCM (Flow Cytometry)||1/200 - 1/400|
|WB (Western Blot)||1/500 - 1/2000|
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Figure 1: Western blot analysis using AIF mAb against human AIF (AA: 1-261) recombinant protein. (Expected MW is 35.6 kDa)
Figure 2: Western blot analysis using AIF mouse mAb against NIH/3T3 (1), Jurkat (2), Hela (3), HepG2 (4), MOLT4 (5), C6 (6), RAJI (7), Cos7 (8) and PC-12 (9) cell lysate.
Figure 3: Immunohistochemical analysis of paraffin-embedded human breast cancer tissues using AIF mouse mAb with DAB staining.
Figure 4: Immunohistochemical analysis of paraffin-embedded human cervical cancer tissues using AIF mouse mAb with DAB staining.
Figure 5: Immunohistochemical analysis of paraffin-embedded human rectum cancer tissues using AIF mouse mAb with DAB staining.
Figure 6: Immunohistochemical analysis of paraffin-embedded human lung cancer tissues using AIF mouse mAb with DAB staining.
Figure 7: Immunofluorescence analysis of NIH/3T3 cells using AIF mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
Figure 8: Flow cytometric analysis of HepG2 cells using AIF mouse mAb (blue) and negative control (red).
Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);
This gene encodes a flavoprotein essential for nuclear disassembly in apoptotic cells, and it is found in the mitochondrial intermembrane space in healthy cells. Induction of apoptosis results in the translocation of this protein to the nucleus where it affects chromosome condensation and fragmentation. In addition, this gene product induces mitochondria to release the apoptogenic proteins cytochrome c and caspase-9. Mutations in this gene cause combined oxidative phosphorylation deficiency 6, which results in a severe mitochondrial encephalomyopathy. Alternative splicing results in multiple transcript variants. A related pseudogene has been identified on chromosome 10
|References (references)||1. Apoptosis. 2009 Jun;14(6):796-808. |
2. PLoS One. 2009;4(2):e4394. Epub 2009 Feb 6.