AFP Primary Antibody

Item Information
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Description

AFP (alpha-fetoprotein), a major plasma protein produced by the yolk sac and the liver during fetal life. Alpha-fetoprotein expression in adults is often associated with hepatoma or teratoma. However, hereditary persistance of alpha-fetoprotein may also be found in individuals with no obvious pathology. The protein is thought to be the fetal counterpart of serum albumin, and the alpha-fetoprotein and albumin genes are present in tandem in the same transcriptional orientation on chromosome 4. Alpha-fetoprotein is found in monomeric as well as dimeric and trimeric forms, and binds copper, nickel, fatty acids and bilirubin. The level of alpha-fetoprotein in amniotic fluid is used to measure renal loss of protein to screen for spina bifida and anencephaly.

Product Overview
Entrez GenelD
174
Aliases
alpha-fetoprotein; FETA; HPAFP
Clone#
6E6
Host / Isotype
Mouse / IgG2b
Species Reactivity
Human
Immunogen
Purified recombinant fragment of human AFP expressed in E. Coli.
Formulation
Ascitic fluid containing 0.03% sodium azide.
Storage
4°C; -20°C for long term storage
Product Applications
WB (Western Blot)
1/500 - 1/2000
ELISA
1/10000
References
1. Proc Natl Acad Sci U S A. 1985 Nov;82(21):7160-4.
2. Pediatr Hematol Oncol. 2001 Dec;18(8):509-18.
3. Tumour Biol. 2002 Jul-Aug;23(4):202-11.
Product Image
Western Blot
Figure 1: Western blot analysis using AFP mouse mAb against HepG2 (1) and SMMC-7721 (2) cell lysate.
Figure 1: Western blot analysis using AFP mouse mAb against HepG2 (1) and SMMC-7721 (2) cell lysate.
For Research Use Only. Not for use in diagnostic procedures.