ADAR Primary Antibody

Item Information
Catalog # Size/Concentration Price
31197 100μl $341.00
Specification
AliasesDSH; AGS6; G1P1; IFI4; P136; ADAR1; DRADA; DSRAD; IFI-4; K88DSRBP
Product OrderA
Clone#4E2E4
Entrez GeneID103
FormulationPurified antibody in PBS with 0.05% sodium azide
HostMouse
IsotypeIgG1
ImmunogenPurified recombinant fragment of human ADAR (AA: 1085-1223) expressed in E. Coli.
MW136kDa
Shipping InformationThis product will ship in a box containing blue ice at a temperature of 4°C.  Learn More
Species ReactivityHuman
Application
ELISA1/10000
ICC (Immunocytochemistry)1/200 - 1/1000
FCM (Flow Cytometry)1/200 - 1/400
Sequence
1085-1223
Catalog
31197
$341.00
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Images

Elisa

Cell Culture Products
Figure 1: Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)

Western Blot

Cell Culture Products
Figure 2:Western blot analysis using ADAR mAb against human ADAR (AA: 1085-1223) recombinant protein. (Expected MW is 42.1 kDa)

Western Blot

Cell Culture Products
Figure 3:Western blot analysis using ADAR mAb against HEK293 (1) and ADAR (AA: 1085-1223)-hIgGFc transfected HEK293 (2) cell lysate.

Immunofluorescence analysis

Cell Culture Products
Figure 4:Immunofluorescence analysis of Hela cells using ADAR mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)

Flow cytometric

Cell Culture Products
Figure 5:Flow cytometric analysis of Hela cells using ADAR mouse mAb (green) and negative control (red).

Flow cytometric

Cell Culture Products
Figure 6:Flow cytometric analysis of Jurkat cells using ADAR mouse mAb (green) and negative control (red).
Product Overview
Description This gene encodes the enzyme responsible for RNA editing by site-specific deamination of adenosines. This enzyme destabilizes double-stranded RNA through conversion of adenosine to inosine. Mutations in this gene have been associated with dyschromatosis symmetrica hereditaria. Alternative splicing results in multiple transcript variants.
References (references)
References (references) 1.Cell Res. 2015 Apr;25(4):459-76.
2.PLoS One. 2014 Oct 1;9(10):e108476.

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