ACVR1 Primary Antibody
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Description
Activins are dimeric growth and differentiation factors which belong to the transforming growth factor-beta (TGF-beta) superfamily of structurally related signaling proteins. Activins signal through a heteromeric complex of receptor serine kinases which include at least two type I ( I and IB) and two type II (II and IIB) receptors. These receptors are all transmembrane proteins, composed of a ligand-binding extracellular domain with cysteine-rich region, a transmembrane domain, and a cytoplasmic domain with predicted serine/threonine specificity. Type I receptors are essential for signaling; and type II receptors are required for binding ligands and for expression of type I receptors. Type I and II receptors form a stable complex after ligand binding, resulting in phosphorylation of type I receptors by type II receptors. This gene encodes activin A type I receptor which signals a particular transcriptional response in concert with activin type II receptors. Mutations in this gene are associated with fibrodysplasia ossificans progressive.
Product Overview
Immunogen
Purified recombinant fragment of human ACVR1 (AA: 21-120) expressed in E. Coli.
Formulation
Purified antibody in PBS with 0.05% sodium azide
Storage
4°C; -20°C for long term storage
Product Image
Elisa

Figure 1: Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
Western Blot

Figure 2:Western blot analysis using ACVR1 mAb against human ACVR1 (AA: 21-120) recombinant protein. (Expected MW is 37.1 kDa)
Western Blot

Figure 3:Western blot analysis using ACVR1 mAb against HEK293 (1) and ACVR1 (AA: 21-120)-hIgGFc transfected HEK293 (2) cell lysate.
Immunofluorescence analysis

Figure 4:Immunofluorescence analysis of Hela cells using ACVR1 mouse mAb. Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin.
Immunofluorescence analysis

Figure 5:Immunofluorescence analysis of Hela cells using ACVR1 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)
Flow cytometric

Figure 6:Flow cytometric analysis of Hela cells using ACVR1 mouse mAb (green) and negative control (red).
Immunohistochemical analysis

Figure 7:Immunohistochemical analysis of paraffin-embedded ovarian cancer tissues using ACVR1 mouse mAb with DAB staining.
Immunohistochemical analysis

Figure 8:Immunohistochemical analysis of paraffin-embedded endometrial cancer tissues using ACVR1 mouse mAb with DAB staining.
For Research Use Only. Not for use in diagnostic procedures.