ABCG5 Primary Antibody

Item Information
Catalog #Size/ConcentrationPrice
Specification
AliasesSTSL
ProductOrderA
Clone#1B5E10
Entrez GenelD64240
FormulationPurified antibody in PBS with 0.05% sodium azide
HostMouse
IsotypeIgG1
ImmunogenPurified recombinant fragment of human ABCG5 (AA: 306-367) expressed in E. Coli.
MW72.5kDa
Shipping InformationThis product will ship in a box containing blue ice at a temperature of 4°C. Learn More
Species ReactivityHuman
Application
ELISA1/10000
IHC_P(Immunohistochemistry)1/200 - 1/1000
FCM (Flow Cytometry)1/200 - 1/400
WB (Western Blot)1/500 - 1/2000
Sequence
306-367
Catalog#: 30518
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Images
Western Blot
Figure 1: Western blot analysis using ABCG5 mAb against human ABCG5 recombinant protein. (Expected MW is 32.7 kDa)
Figure 1: Western blot analysis using ABCG5 mAb against human ABCG5 recombinant protein. (Expected MW is 32.7 kDa)
Western Blot
Figure 2: Western blot analysis using ABCG5 mAb against HEK293 (1) and ABCG5 (AA: 306-367)-hIgGFc transfected HEK293 (2) cell lysate.
Figure 2: Western blot analysis using ABCG5 mAb against HEK293 (1) and ABCG5 (AA: 306-367)-hIgGFc transfected HEK293 (2) cell lysate.
Western Blot
Figure 3: Western blot analysis using ABCG5 mouse mAb against HL7702 (1), RAJI (2) and Jurkat (3) cell lysate.
Figure 3: Western blot analysis using ABCG5 mouse mAb against HL7702 (1), RAJI (2) and Jurkat (3) cell lysate.
Flow cytometric
Figure 4: Flow cytometric analysis of A549 cells using ABCG5 mouse mAb (green) and negative control (red).
Figure 4: Flow cytometric analysis of A549 cells using ABCG5 mouse mAb (green) and negative control (red).
Immunohistochemical analysis
Figure 5: Immunohistochemical analysis of paraffin-embedded cervical cancer tissues using ABCG5 mouse mAb with DAB staining.
Figure 5: Immunohistochemical analysis of paraffin-embedded cervical cancer tissues using ABCG5 mouse mAb with DAB staining.
Immunohistochemical analysis
Figure 6: Immunohistochemical analysis of paraffin-embedded rectum cancer tissues using ABCG5 mouse mAb with DAB staining.
Figure 6: Immunohistochemical analysis of paraffin-embedded rectum cancer tissues using ABCG5 mouse mAb with DAB staining.
Elisa
Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);
Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);
Product Overview
DescriptionThe protein encoded by this gene is a member of the superfamily of ATP-binding cassette (ABC) transporters. ABC proteins transport various molecules across extra- and intra-cellular membranes. ABC genes are divided into seven distinct subfamilies (ABC1, MDR/TAP, MRP, ALD, OABP, GCN20, White). This protein is a member of the White subfamily. The protein encoded by this gene functions as a half-transporter to limit intestinal absorption and promote biliary excretion of sterols. It is expressed in a tissue-specific manner in the liver, colon, and intestine. This gene is tandemly arrayed on chromosome 2, in a head-to-head orientation with family member ABCG8. Mutations in this gene may contribute to sterol accumulation and atheroschlerosis, and have been observed in patients with sitosterolemia.

References (references)
References (references)1. PLoS One. 2012;7(5):e37972.
2. Biochemistry. 2010 Apr 27;49(16):3403-11.