ABCG5 Primary Antibody
Item Information
Catalog #
Size
Price
Description
The protein encoded by this gene is a member of the superfamily of ATP-binding cassette (ABC) transporters. ABC proteins transport various molecules across extra- and intra-cellular membranes. ABC genes are divided into seven distinct subfamilies (ABC1, MDR/TAP, MRP, ALD, OABP, GCN20, White). This protein is a member of the White subfamily. The protein encoded by this gene functions as a half-transporter to limit intestinal absorption and promote biliary excretion of sterols. It is expressed in a tissue-specific manner in the liver, colon, and intestine. This gene is tandemly arrayed on chromosome 2, in a head-to-head orientation with family member ABCG8. Mutations in this gene may contribute to sterol accumulation and atheroschlerosis, and have been observed in patients with sitosterolemia.
Product Overview
Entrez GenelD
64240
Aliases
STSL
Clone#
1B5E10
Host / Isotype
Mouse / IgG1
Species Reactivity
Human
Immunogen
Purified recombinant fragment of human ABCG5 (AA: 306-367) expressed in E. Coli.
Formulation
Purified antibody in PBS with 0.05% sodium azide
Storage
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Product Applications
WB (Western Blot)
1/500 - 1/2000
IHC_P(Immunohistochemistry)
1/200 - 1/1000
FCM (Flow Cytometry)
1/200 - 1/400
ELISA
1/10000
References
1. PLoS One. 2012;7(5):e37972.
2. Biochemistry. 2010 Apr 27;49(16):3403-11.
2. Biochemistry. 2010 Apr 27;49(16):3403-11.
Product Image
Western Blot
Figure 1: Western blot analysis using ABCG5 mAb against human ABCG5 recombinant protein. (Expected MW is 32.7 kDa)
Western Blot
Figure 2: Western blot analysis using ABCG5 mAb against HEK293 (1) and ABCG5 (AA: 306-367)-hIgGFc transfected HEK293 (2) cell lysate.
Western Blot
Figure 3: Western blot analysis using ABCG5 mouse mAb against HL7702 (1), RAJI (2) and Jurkat (3) cell lysate.
Flow cytometric
Figure 4: Flow cytometric analysis of A549 cells using ABCG5 mouse mAb (green) and negative control (red).
Immunohistochemical analysis
Figure 5: Immunohistochemical analysis of paraffin-embedded cervical cancer tissues using ABCG5 mouse mAb with DAB staining.
Immunohistochemical analysis
Figure 6: Immunohistochemical analysis of paraffin-embedded rectum cancer tissues using ABCG5 mouse mAb with DAB staining.
Elisa
Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);
For Research Use Only. Not for use in diagnostic procedures.