ABCG2 Primary Antibody

Item Information

Catalog #

Size

Price

Description

The membrane-associated protein encoded by this gene is included in the superfamily of ATP-binding cassette (ABC) transporters. ABC proteins transport various molecules across extra- and intra-cellular membranes. ABC genes are divided into seven distinct subfamilies (ABC1, MDR/TAP, MRP, ALD, OABP, GCN20, White). This protein is a member of the White subfamily. Alternatively referred to as a breast cancer resistance protein, this protein functions as a xenobiotic transporter which may play a major role in multi-drug resistance. It likely serves as a cellular defense mechanism in response to mitoxantrone and anthracycline exposure. Significant expression of this protein has been observed in the placenta, which may suggest a potential role for this molecule in placenta tissue.Tissue specificity: Highly expressed in placenta. Low expression in small intestine, liver and colon.

Product Overview

Entrez GenelD

9429

Aliases

MRX; MXR; ABCP; BCRP; BMDP; MXR1; ABC15; BCRP1; CD338; CDw338; EST157481; MGC102821

Clone#

1H2

Host / Isotype

Mouse / IgG1

Species Reactivity

Human, Mouse, Monkey

Immunogen

Purified recombinant fragment of human ABCG2 expressed in E. Coli.

Formulation

Ascitic fluid containing 0.03% sodium azide.

Storage

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.

Product Applications

WB (Western Blot)

1/500 - 1/2000

ICC (Immunocytochemistry)

1/200 - 1/1000

ELISA

1/10000

References

1. Carcinogenesis. 2008 Dec;29(12):2289-97.
2. Pharm Res. 2009 Feb;26(2):449-58.

Product Image

Western Blot

Figure 1: Western blot analysis using ABCG2 mouse mAb against HepG2 (1), Cos7 (2), Jurkat (3) and NIH/3T3 (4) cell lysate.

Immunofluorescence analysis

Figure 2: Immunofluorescence analysis of Hela cells using ABCG2 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.

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