ABCB1 Primary Antibody
Item Information
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Price
Description
The membrane-associated protein encoded by this gene is a member of the superfamily of ATP-binding cassette (ABC) transporters. ABC proteins transport various molecules across extra- and intra-cellular membranes. ABC genes are divided into seven distinct subfamilies (ABC1, MDR/TAP, MRP, ALD, OABP, GCN20, White). This protein is a member of the MDR/TAP subfamily. Members of the MDR/TAP subfamily are involved in multidrug resistance. The protein encoded by this gene is an ATP-dependent drug efflux pump for xenobiotic compounds with broad substrate specificity. It is responsible for decreased drug accumulation in multidrug-resistant cells and often mediates the development of resistance to anticancer drugs. This protein also functions as a transporter in the blood-brain barrier.
Product Overview
Entrez GenelD
5243
Aliases
CLCS; MDR1; P-GP; PGY1; ABC20; CD243; GP170
Clone#
6G11C12
Host / Isotype
Mouse / IgG1
Species Reactivity
Human
Immunogen
Purified recombinant fragment of human ABCB1 (AA: 632-693) expressed in E. Coli.
Formulation
Purified antibody in PBS with 0.05% sodium azide
Storage
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Product Applications
WB (Western Blot)
1/500 - 1/2000
ELISA
1/10000
References
1. Pharmacol Rep. 2012;64(6):1560-6.
2. J Cancer Res Ther. 2012 Apr-Jun;8(2):226-31.
2. J Cancer Res Ther. 2012 Apr-Jun;8(2):226-31.
Product Image
Western Blot
Figure 1: Western blot analysis using ABCB1 mAb against human ABCB1 (AA: 632-693) recombinant protein. (Expected MW is 32.4 kDa)
Western Blot
Figure 2: Western blot analysis using ABCB1 mAb against HEK293 (1) and ABCB1 (AA: 632-693)-hIgGFc transfected HEK293 (2) cell lysate.
Elisa
Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);
For Research Use Only. Not for use in diagnostic procedures.