WT1 Primary Antibody

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Description

This gene encodes a transcription factor that contains four zinc-finger motifs at the C-terminus and a proline/glutamine-rich DNA-binding domain at the N-terminus. It has an essential role in the normal development of the urogenital system, and it is mutated in a small subset of patients with Wilms tumor. This gene exhibits complex tissue-specific and polymorphic imprinting pattern, with biallelic, and monoallelic expression from the maternal and paternal alleles in different tissues. Multiple transcript variants have been described. In several variants, there is evidence for the use of a non-AUG (CUG) translation initiation codon upstream of, and in-frame with the first AUG. Authors of PMID:7926762 also provide evidence that WT1 mRNA undergoes RNA editing in human and rat, and that this process is tissue-restricted and developmentally regulated.

Product Overview
Entrez GenelD
7490
Aliases
GUD; AWT1; WAGR; WT33; NPHS4; WIT-2
Clone#
7F9G7H2
Host / Isotype
Mouse / Mouse IgG1
Immunogen
Purified recombinant fragment of human WT1 (AA: 1-181) expressed in E. Coli.
Formulation
Purified antibody in PBS with 0.05% sodium azide
Storage
4°C; -20°C for long term storage
Product Applications
WB (Western Blot)
1/500 - 1/2000
FCM (Flow Cytometry)
1/200-1/400
ELISA
1/10000
References
1.Indian J Cancer. 2019 Jul-Sep;56(3):197-201. 2.Br J Cancer. 2018 Dec;119(12):1508-1517.
Product Image
ELISA
Figure 1: Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng)
Figure 1: Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng)
WESTERN BLOT
Figure 2: Western blot analysis using WT1 mAb against human WT1 (AA: 1-181) recombinant protein. (Expected MW is 21.2 kDa)
Figure 2: Western blot analysis using WT1 mAb against human WT1 (AA: 1-181) recombinant protein. (Expected MW is 21.2 kDa)
WESTERN BLOT
Figure 3: Western blot analysis using WT1 mAb against HEK293 (1) and WT1 (AA: 1-181)-hIgGFc transfected HEK293 (2) cell lysate.
Figure 3: Western blot analysis using WT1 mAb against HEK293 (1) and WT1 (AA: 1-181)-hIgGFc transfected HEK293 (2) cell lysate.
WESTERN BLOT
Figure 4: Western blot analysis using WT1 mouse mAb against K562 (1), COS7 (2), SK-OV-3 (3), Hela (4), and PC-3 (5) cell lysate.
Figure 4: Western blot analysis using WT1 mouse mAb against K562 (1), COS7 (2), SK-OV-3 (3), Hela (4), and PC-3 (5) cell lysate.
FLOW CYTOMETRY
Figure 5: Flow cytometric analysis of Hela cells using WT1 mouse mAb (green) and negative control (red).
Figure 5: Flow cytometric analysis of Hela cells using WT1 mouse mAb (green) and negative control (red).
For Research Use Only. Not for use in diagnostic procedures.

Catalog#: 31886

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