Mouse Monoclonal Antibody to TRIB2

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Description

This gene encodes one of three members of the Tribbles family. The Tribbles members share a Trb domain, which is homologous to protein serine-threonine kinases, but lacks the active site lysine and probably lacks a catalytic function. The Tribbles proteins interact and modulate the activity of signal transduction pathways in a number of physiological and pathological processes. This Tribbles member induces apoptosis of cells mainly of the hematopoietic origin. It has been identified as a protein up-regulated by inflammatory stimuli in myeloid (THP-1) cells, and also as an oncogene that inactivates the transcription factor C/EBPalpha (CCAAT/enhancer-binding protein alpha) and causes acute myelogenous leukemia. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Mar 2009]

Product Overview
Entrez GenelD
28951
Aliases
C5FW; TRB2; GS3955
Clone#
6A12A12B1
Host / Isotype
Mouse / IgG2a
Immunogen
Purified recombinant fragment of human TRIB2 (AA: 1-200) expressed in E. Coli.
Formulation
Purified antibody in PBS with 0.05% sodium azide
Storage
4℃; -20℃ for long term storage
Product Applications
WB (Western Blot)
1/500 - 1/2000
ICC (Immunocytochemistry)
1/200 - 1/1000
FCM (Flow Cytometry)
1/200 - 1/400
ELISA
1/10000
References
1.Exp Mol Med.2017 Nov 24;49(11):e401.2.Life Sci.2020 Feb 15;243:117323.
Product Image
Elisa
Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
Western Blot
Figure 2:Western blot analysis using TRIB2 mAb against human TRIB2 (AA:1-200) recombinant protein. (Expected MW is 25.7 kDa)
Figure 2:Western blot analysis using TRIB2 mAb against human TRIB2 (AA:1-200) recombinant protein. (Expected MW is 25.7 kDa)
Western Blot
Figure 3:Western blot analysis using TRIB2 mAb against HEK293 (1) and TRIB2 (AA:1-200)-hIgGFc transfected HEK293 (2) cell lysate.
Figure 3:Western blot analysis using TRIB2 mAb against HEK293 (1) and TRIB2 (AA:1-200)-hIgGFc transfected HEK293 (2) cell lysate.
Western Blot
Figure 4:Western blot analysis using TRIB2 mouse mAb against Jurkat (1), MCF-7 (2), SW480 (3),and A375 (4) cell lysate.
Figure 4:Western blot analysis using TRIB2 mouse mAb against Jurkat (1), MCF-7 (2), SW480 (3),and A375 (4) cell lysate.
Immunofluorescence analysis
Figure 5:Immunofluorescence analysis of Hela cells using TRIB2 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)
Figure 5:Immunofluorescence analysis of Hela cells using TRIB2 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)
Flow cytometric analysis
Figure 6:Flow cytometric analysis of Jurkat cells using TRIB2 mouse mAb (green) and negative control (red).
Figure 6:Flow cytometric analysis of Jurkat cells using TRIB2 mouse mAb (green) and negative control (red).
Flow cytometric analysis
Figure 7:Flow cytometric analysis of K562 cells using TRIB2 mouse mAb (green) and negative control (red).
Figure 7:Flow cytometric analysis of K562 cells using TRIB2 mouse mAb (green) and negative control (red).
For Research Use Only. Not for use in diagnostic procedures.

Catalog#: 32238

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