Mouse Monoclonal Antibody to TRIB2

Item Information

Catalog #

Size

Price

Description

This gene encodes one of three members of the Tribbles family. The Tribbles members share a Trb domain, which is homologous to protein serine-threonine kinases, but lacks the active site lysine and probably lacks a catalytic function. The Tribbles proteins interact and modulate the activity of signal transduction pathways in a number of physiological and pathological processes. This Tribbles member induces apoptosis of cells mainly of the hematopoietic origin. It has been identified as a protein up-regulated by inflammatory stimuli in myeloid (THP-1) cells, and also as an oncogene that inactivates the transcription factor C/EBPalpha (CCAAT/enhancer-binding protein alpha) and causes acute myelogenous leukemia. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Mar 2009]

Product Overview

Entrez GenelD

28951

Aliases

C5FW; TRB2; GS3955

Clone#

6A12A12

Host / Isotype

Mouse / IgG2a

Immunogen

Purified recombinant fragment of human TRIB2 (AA: 1-200) expressed in E. Coli.

Formulation

Purified antibody in PBS with 0.05% sodium azide

Storage

4℃; -20℃ for long term storage

Product Applications

WB (Western Blot)

1/500 - 1/2000

IHC_P(Immunohistochemistry)

1/200 - 1/1000

ICC (Immunocytochemistry)

1/200 - 1/1000

FCM (Flow Cytometry)

1/200 - 1/400

ELISA

1/10000

References

1,Int J Cancer. 2017 Oct 15;141(8):1600-1614. 2,Exp Mol Med. 2017 Nov 24;49(11):e401.

Product Image

Elisa

Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)

Western Blot

Figure 2:Western blot analysis using TRIB2 mAb against human TRIB2 (AA: 1-200) recombinant protein. (Expected MW is 25.7 kDa)

Western Blot

Figure 3:Western blot analysis using TRIB2 mAb against HEK293-6e (1) and human TRIB2 (AA: 1-200)-hIgGFc transfected HEK293-6e (2) cell lysate.

Flow cytometric analysis

Figure 4:Flow cytometric analysis of Jurkat cells using TRIB2 mouse mAb (green) and negative control (red).

Immunohistochemical analysis

Figure 5:Immunohistochemical analysis of paraffin-embedded bladder cancer tissues using TRIB2 mouse mAb with DAB staining.

Immunohistochemical analysis

Figure 6:Immunohistochemical analysis of paraffin-embedded cervical cancer tissues using TRIB2 mouse mAb with DAB staining.

Immunofluorescence analysis

Figure 7:Immunofluorescence analysis of Hela cells using TRIB2 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)

For Research Use Only. Not for use in diagnostic procedures.