Mouse Monoclonal Antibody to TLR10

Item Information

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Description

The protein encoded by this gene is a member of the Toll-like receptor (TLR) family which plays a fundamental role in pathogen recognition and activation of innate immunity. TLRs are highly conserved from Drosophila to humans and share structural and functional similarities. They recognize pathogen-associated molecular patterns (PAMPs) that are expressed on infectious agents, and mediate the production of cytokines necessary for the development of effective immunity. The various TLRs exhibit different patterns of expression. This gene is most highly expressed in lymphoid tissues such as spleen, lymph node, thymus, and tonsil. Multiple alternatively spliced transcript variants which encode different protein isoforms have been found for this gene. [provided by RefSeq, Aug 2010]

Product Overview

Entrez GenelD

81793

Aliases

CD290

Clone#

2A10A4

Host / Isotype

Mouse / IgG2a

Immunogen

Purified recombinant fragment of human TLR10 (AA: extra(20-219)) expressed in E. Coli.

Formulation

Purified antibody in PBS with 0.05% sodium azide

Storage

4℃; -20℃ for long term storage

Product Applications

WB (Western Blot)

1/500 - 1/2000

ICC (Immunocytochemistry)

1/200 - 1/1000

FCM (Flow Cytometry)

1/200 - 1/400

References

1,Cytokine. 2018 Aug;108:205-212. 2,APMIS. 2020 Apr;128(4):335-342.

Product Image

Western Blot

Figure 1:Western blot analysis using TLR10 mAb against human TLR10 (AA: extra(20-219)) recombinant protein. (Expected MW is 26 kDa)

Western Blot

Figure 2:Western blot analysis using TLR10 mAb against HEK293-6e (1) and TLR10 (AA: extra(20-219))-hIgGFc transfected HEK293-6e (2) cell lysate.

Flow cytometric analysis

Figure 3:Flow cytometric analysis of Jurkat cells using TLR10 mouse mAb (green) and negative control (red).

Flow cytometric analysis

Figure 4:Flow cytometric analysis of HL-60 cells using TLR10 mouse mAb (green) and negative control (red).

Immunofluorescence analysis

Figure 5:Immunofluorescence analysis of Hela cells using TLR10 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)

For Research Use Only. Not for use in diagnostic procedures.