Mouse Monoclonal Antibody to KCNN4

Item Information

Catalog #

Size

Price

Description

The protein encoded by this gene is part of a potentially heterotetrameric voltage-independent potassium channel that is activated by intracellular calcium. Activation is followed by membrane hyperpolarization, which promotes calcium influx. The encoded protein may be part of the predominant calcium-activated potassium channel in T-lymphocytes. This gene is similar to other KCNN family potassium channel genes, but it differs enough to possibly be considered as part of a new subfamily.

Product Overview

Entrez GenelD

3783

Aliases

IK; IK1; SK4; DHS2; KCA4; hSK4; IKCA1; hKCa4; KCa3.1; hIKCa1

Clone#

1B4B4

Host / Isotype

Mouse / IgG1

Immunogen

Purified recombinant fragment of human KCNN4 (AA: extra 286-427) expressed in E. Coli.

Formulation

Purified antibody in PBS with 0.05% sodium azide

Storage

4℃; -20℃ for long term storage

Product Applications

WB (Western Blot)

1/500 - 1/2000

ICC (Immunocytochemistry)

1/50 - 1/200

FCM (Flow Cytometry)

1/200 - 1/400

ELISA

1/10000

References

1.Exp Cell Res. 2018 Aug 15;369(2):208-217. 2.Sci Signal. 2018 Apr 24;11(527):eaaq1616.

Product Image

Elisa

Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)

Western Blot

Figure 2:Western blot analysis using KCNN4 mAb against human KCNN4 (AA: extra 286-427) recombinant protein. (Expected MW is 42.7 kDa)

Western Blot

Figure 3:Western blot analysis using KCNN4 mAb against HEK293-6e (1) and KCNN4 (AA: extra 286-427)-hIgGFc transfected HEK293-6e (2) cell lysate.

Immunofluorescence analysis

Figure 4:Immunofluorescence analysis of Hela cells using KCNN4 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)

Flow cytometric analysis